BSEP Antibody (F-6): sc-74500

BSEP Antibody (F-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects BSEP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BSEP (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Bile Salt Export Pump (BSEP), also known as ABCB11, plays a crucial role in the transport of bile acids across the hepatocyte canalicular membrane, which is essential for bile formation and regulation of bile acid homeostasis. BSEP is located in the liver, where BSEP facilitates the secretion of bile salts from the bloodstream into bile, thereby preventing toxic accumulation of bile acids in the liver. Proper functioning of BSEP is vital for digestion and absorption of dietary fats and fat-soluble vitamins, and BSEP dysfunction can lead to cholestasis and liver diseases. Additionally, BSEP contains potential phosphorylation sites for various kinases, indicating that BSEP activity may be modulated by post-translational modifications in response to bile salt concentrations, further underscoring BSEP importance in maintaining liver health and metabolic balance.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BSEP Antibody (F-6) References:

1. Hepatic transport of bile salts.  |  Kullak-Ublick, GA., et al. 2000. Semin Liver Dis. 20: 273-92. PMID: 11076396
2. Characterization of bile acid transport mediated by multidrug resistance associated protein 2 and bile salt export pump.  |  Akita, H., et al. 2001. Biochim Biophys Acta. 1511: 7-16. PMID: 11248200
3. Characterization of the mouse bile salt export pump overexpressed in the baculovirus system.  |  Noe, J., et al. 2001. Hepatology. 33: 1223-31. PMID: 11343252
4. Human bile salt export pump promoter is transactivated by the farnesoid X receptor/bile acid receptor.  |  Ananthanarayanan, M., et al. 2001. J Biol Chem. 276: 28857-65. PMID: 11387316
5. Semi quantitative expression analysis of MDR3, FIC1, BSEP, OATP-A, OATP-C,OATP-D, OATP-E and NTCP gene transcripts in 1st and 3rd trimester human placenta.  |  Patel, P., et al. 2003. Placenta. 24: 39-44. PMID: 12495658
6. Impaired localisation and transport function of canalicular Bsep in taurolithocholate induced cholestasis in the rat.  |  Crocenzi, FA., et al. 2003. Gut. 52: 1170-7. PMID: 12865277
7. BSEP and MDR3 haplotype structure in healthy Caucasians, primary biliary cirrhosis and primary sclerosing cholangitis.  |  Pauli-Magnus, C., et al. 2004. Hepatology. 39: 779-91. PMID: 14999697
8. LPS-induced downregulation of MRP2 and BSEP in human liver is due to a posttranscriptional process.  |  Elferink, MG., et al. 2004. Am J Physiol Gastrointest Liver Physiol. 287: G1008-16. PMID: 15205115
9. The bile salt export pump: molecular properties, function and regulation.  |  Arrese, M. and Ananthanarayanan, M. 2004. Pflugers Arch. 449: 123-31. PMID: 15578267
10. Severe bile salt export pump deficiency: 82 different ABCB11 mutations in 109 families.  |  Strautnieks, SS., et al. 2008. Gastroenterology. 134: 1203-14. PMID: 18395098
11. The bile salt export pump (BSEP) in health and disease.  |  Kubitz, R., et al. 2012. Clin Res Hepatol Gastroenterol. 36: 536-53. PMID: 22795478
12. Genotype correlates with the natural history of severe bile salt export pump deficiency.  |  van Wessel, DBE., et al. 2020. J Hepatol. 73: 84-93. PMID: 32087350

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BSEP Antibody (F-6) has 60 citations in a variety of scientific publications.

Primary antibodies like Anti-BSEP Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BSEP Antibody (F-6) Specifications

Alternate Product Name: BSEP

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-180

Epitope Target: BSEP

Epitope Species: human

Product Reactivity/Detection: BSEP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 60 citations in various scientific publications

Available Conjugates/Forms for BSEP Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin