Brn-3b/BRN3B/POU4F2 CRISPR/Cas9 KO Plasmid (h): sc-400382

Overview

POU4F2 (Brn-3b/BRN3B) encodes a POU-domain transcription factor that regulates cell type–specific gene expression programs, with prominent roles in neuronal differentiation and maintenance of sensory neuron identity, particularly in retinal ganglion cells. By binding octamer-like DNA motifs, BRN3B coordinates transcriptional networks controlling development, survival, and stress responses, interfacing with broader transcriptional regulation pathways that shape cell fate decisions. Altered POU4F2 activity has been linked to dysregulated differentiation and context-dependent changes in proliferation and apoptosis, making it relevant to studies of neurodevelopmental and neurodegenerative mechanisms as well as cancer-associated transcriptional rewiring. As a lineage-defining factor, it is frequently used as a marker and functional node in models that interrogate neuronal identity and stimulus-dependent transcription.

Brn-3b/BRN3B/POU4F2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the POU4F2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the POU4F2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the POU4F2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Brn-3b/BRN3B/POU4F2 protein expression.

This CRISPR knockout system enables efficient generation of POU4F2-deficient cell models for investigation of Brn-3b/BRN3B/POU4F2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting POU4F2 exon(s) critical for Brn-3b/BRN3B/POU4F2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple POU4F2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Brn-3b/BRN3B/POU4F2 CRISPR/Cas9 KO Plasmid (h) and Brn-3b/BRN3B/POU4F2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the POU4F2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Brn-3b/BRN3B/POU4F2 HDR Plasmid (h) and Brn-3b/BRN3B/POU4F2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by POU4F2 homology arms to support homology-directed repair at defined POU4F2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.