Brn-3b/BRN3B/POU4F2 Antibody (D-8): sc-514474

Brn-3b Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Brn-3b protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Brn-3b (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Brn-3b protein, a member of the POU family of transcription factors, plays a crucial role in the development of the central nervous system by regulating gene expression in a highly restricted subset of neurons. Notably, anti-Brn-3b antibody (D-8) targets a protein that functions primarily as a transcriptional repressor, distinguishing Brn-3b from counterparts Brn-3a and Brn-3c, which are known to stimulate transcription. This unique regulatory role is vital for proper expression of genes involved in neuronal differentiation and function. Additionally, Brn-3b has been shown to specifically regulate acetylcholine receptor expression, highlighting Brn-3b′s importance in synaptic transmission and neuromuscular junction formation. Understanding Brn-3b function is essential for elucidating molecular mechanisms underlying neuronal development and potential implications in neurodegenerative diseases.

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Brn-3b/BRN3B/POU4F2 Antibody (D-8) References:

1. A novel POU homeodomain gene specifically expressed in cells of the developing mammalian nervous system.  |  Collum, RG., et al. 1992. Nucleic Acids Res. 20: 4919-25. PMID: 1357630
2. Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression.  |  Budhram-Mahadeo, VS., et al. 2006. Nucleic Acids Res. 34: 6640-52. PMID: 17145718
3. First Report of Postharvest Fruit Rot on Citrus reticulata Blanco Caused by Fusarium concentricum in China.  |  Xiao, L., et al. 2022. Plant Dis.. PMID: 35984392
4. Correction: Proliferation-associated Brn-3b transcription factor can activate cyclin D1 expression in neuroblastoma and breast cancer cells.  |  Budhram-Mahadeo, VS., et al. 2023. Oncogene. 42: 782. PMID: 36759573
5. Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2.  |  Schonemann, MD., et al. 1995. Genes Dev. 9: 3122-35. PMID: 8543156
6. Preferential differentiation of P19 mouse embryonal carcinoma cells into smooth muscle cells. Use of retinoic acid and antisense against the central nervous system-specific POU transcription factor Brn-2.  |  Suzuki, T., et al. 1996. Circ Res. 78: 395-404. PMID: 8593698
7. The different activities of the two activation domains of the Brn-3a transcription factor are dependent on the context of the binding site.  |  Budhram-Mahadeo, V., et al. 1996. J Biol Chem. 271: 9108-13. PMID: 8621561
8. POU domain factor Brn-3b is required for the development of a large set of retinal ganglion cells.  |  Gan, L., et al. 1996. Proc Natl Acad Sci U S A. 93: 3920-5. PMID: 8632990
9. Role of transcription factors Brn-3.1 and Brn-3.2 in auditory and visual system development.  |  Erkman, L., et al. 1996. Nature. 381: 603-6. PMID: 8637595
10. Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursors.  |  Fedtsova, NG. and Turner, EE. 1995. Mech Dev. 53: 291-304. PMID: 8645597
11. A single amino acid change converts an inhibitory transcription factor into an activator.  |  Dawson, SJ., et al. 1996. J Biol Chem. 271: 11631-3. PMID: 8662774
12. Mutation in transcription factor POU4F3 associated with inherited progressive hearing loss in humans.  |  Vahava, O., et al. 1998. Science. 279: 1950-4. PMID: 9506947

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Brn-3b/BRN3B/POU4F2 Antibody (D-8) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Brn-3b/BRN3B/POU4F2 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Brn-3b/BRN3B/POU4F2 Antibody (D-8) Specifications

Alternate Product Name: POU4F2

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 227-251

Epitope Target: Brn-3b

Epitope Species: human

Product Reactivity/Detection: Brn-3b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Brn-3b/BRN3B/POU4F2 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Brn-3/BRN3/POU4F antibody is Brn-3/BRN3/POU4F Antibody (A-4): sc-390780.