Brn-3/BRN3/POU4F Antibody (A-4): sc-390780

Brn-3 Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Brn-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Brn-3 antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Brn-3, part of the Brn family of transcription factors, plays a crucial role in early embryonic development of the central nervous system, specifically in a highly restricted subset of neurons. Brn-3 proteins are classified into three distinct types: Brn-3a, Brn-3b, and Brn-3c, each with unique functions; while Brn-3a and Brn-3c stimulate transcription, Brn-3b primarily acts as a transcriptional repressor. Brn-3a contains two functional transactivating domains located at both the amino-terminus and carboxy-terminus, which enhance its ability to regulate gene expression. Brn-3b influences the expression of the acetylcholine receptor, highlighting its importance in neuronal signaling. Understanding the roles and interactions of Brn-3 proteins is essential for elucidating the mechanisms underlying neuronal development and function, making Brn-3 (A-4) antibody a valuable tool for researchers studying neurobiology and related fields.

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Brn-3/BRN3/POU4F Antibody (A-4) References:

1. The Brn-3a transcription factor plays a critical role in regulating human papilloma virus gene expression and determining the growth characteristics of cervical cancer cells.  |  Ndisang, D., et al. 1999. J Biol Chem. 274: 28521-7. PMID: 10497216
2. The Brn-3a transcription factor plays a key role in regulating the growth of cervical cancer cells in vivo.  |  Ndisang, D., et al. 2001. Oncogene. 20: 4899-903. PMID: 11521202
3. A novel POU homeodomain gene specifically expressed in cells of the developing mammalian nervous system.  |  Collum, RG., et al. 1992. Nucleic Acids Res. 20: 4919-25. PMID: 1357630
4. First Report of Postharvest Fruit Rot on Citrus reticulata Blanco Caused by Fusarium concentricum in China.  |  Xiao, L., et al. 2022. Plant Dis.. PMID: 35984392
5. Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2.  |  Schonemann, MD., et al. 1995. Genes Dev. 9: 3122-35. PMID: 8543156
6. Preferential differentiation of P19 mouse embryonal carcinoma cells into smooth muscle cells. Use of retinoic acid and antisense against the central nervous system-specific POU transcription factor Brn-2.  |  Suzuki, T., et al. 1996. Circ Res. 78: 395-404. PMID: 8593698
7. The different activities of the two activation domains of the Brn-3a transcription factor are dependent on the context of the binding site.  |  Budhram-Mahadeo, V., et al. 1996. J Biol Chem. 271: 9108-13. PMID: 8621561
8. POU domain factor Brn-3b is required for the development of a large set of retinal ganglion cells.  |  Gan, L., et al. 1996. Proc Natl Acad Sci U S A. 93: 3920-5. PMID: 8632990
9. Role of transcription factors Brn-3.1 and Brn-3.2 in auditory and visual system development.  |  Erkman, L., et al. 1996. Nature. 381: 603-6. PMID: 8637595
10. Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursors.  |  Fedtsova, NG. and Turner, EE. 1995. Mech Dev. 53: 291-304. PMID: 8645597
11. A single amino acid change converts an inhibitory transcription factor into an activator.  |  Dawson, SJ., et al. 1996. J Biol Chem. 271: 11631-3. PMID: 8662774
12. Mutation in transcription factor POU4F3 associated with inherited progressive hearing loss in humans.  |  Vahava, O., et al. 1998. Science. 279: 1950-4. PMID: 9506947

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Brn-3/BRN3/POU4F Antibody (A-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Brn-3/BRN3/POU4F Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Brn-3/BRN3/POU4F Antibody (A-4) Specifications

Alternate Product Name: BRN3

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 321-400

Epitope Target: Brn-3a

Epitope Species: human

Product Reactivity/Detection: Brn-3a, Brn-3b and Brn-3c

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Brn-3/BRN3/POU4F Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Brn-3a/BRN3A/POU4F1 antibody is Brn-3a/BRN3A/POU4F1 Antibody (14A6): sc-8429.