Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Brn-2/BRN2/POU3F2 CRISPR/Cas9 KO Plasmid (h): sc-400392
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Brn-2/BRN2/POU3F2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Brn-2/BRN2/POU3F2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Brn-2/BRN2/POU3F2 Antibody (B-2): sc-393324
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Brn-2/BRN2/POU3F2 CRISPR/Cas9 KO Plasmid (h) | sc-400392 | 20 µg | $397.00 |
Overview
POU3F2 (Brn-2/BRN2) is a POU-homeodomain transcription factor that binds octamer motifs to regulate gene expression programs controlling lineage specification, neuronal differentiation, and maintenance of cell identity. In the nervous system it contributes to cortical development and neurogenic transcriptional networks, while in neural crest–derived lineages it coordinates differentiation-state transitions. Aberrant POU3F2 activity has been linked to dysregulated transcriptional circuitry in cancer biology, particularly in melanoma where it intersects with MAPK-driven state switching and invasion-associated programs. As a nuclear regulator, BRN2 provides a tractable node for interrogating enhancer–promoter control, chromatin occupancy, and context-dependent transcriptional rewiring in human cells.
Brn-2/BRN2/POU3F2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the POU3F2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the POU3F2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the POU3F2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Brn-2/BRN2/POU3F2 protein expression.
This CRISPR knockout system enables efficient generation of POU3F2-deficient cell models for investigation of Brn-2/BRN2/POU3F2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting POU3F2 exon(s) critical for Brn-2/BRN2/POU3F2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple POU3F2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Brn-2/BRN2/POU3F2 CRISPR/Cas9 KO Plasmid (h) and Brn-2/BRN2/POU3F2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the POU3F2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Brn-2/BRN2/POU3F2 HDR Plasmid (h) and Brn-2/BRN2/POU3F2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by POU3F2 homology arms to support homology-directed repair at defined POU3F2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.