Brn-2/BRN2/POU3F2 Antibody (B-2): sc-393324

Brn-2 Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Brn-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Brn-2 antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Brn-2, also known as POU class 3 homeobox 2 (POU3F2), plays a crucial role in the development of the central nervous system, particularly in regulating neuronal differentiation and migration. Brn-2 is predominantly expressed in a specific subset of neurons during early embryonic development, where Brn-2 is essential for proper formation of the forebrain and radial migration of cortical neurons. Brn-2′s ability to regulate gene expression through POU domain is vital for smooth muscle cell development, highlighting Brn-2′s importance in both neural and non-neural tissues. Additionally, Brn-2 interacts with other transcription factors, such as Brn-1 and Brn-3, which further underscores Brn-2′s role in complex regulatory networks governing neuronal development and function. Multiple format options for Brn-2 (B-2) monoclonal antibody make this reagent a versatile tool for researchers studying intricate processes of neuronal development and differentiation.

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Brn-2/BRN2/POU3F2 Antibody (B-2) References:

1. Cysteine string protein regulates G protein modulation of N-type calcium channels.  |  Magga, JM., et al. 2000. Neuron. 28: 195-204. PMID: 11086994
2. NF-Y binding is required for transactivation of neuronal aromatic L-amino acid decarboxylase gene promoter by the POU-domain protein Brn-2.  |  Dugast, C. and Weber, MJ. 2001. Brain Res Mol Brain Res. 89: 58-70. PMID: 11311976
3. Transcriptional regulation of cortical neuron migration by POU domain factors.  |  McEvilly, RJ., et al. 2002. Science. 295: 1528-32. PMID: 11859196
4. Isolation of the human genomic brain-2/N-Oct 3 gene (POUF3) and assignment to chromosome 6q16.  |  Atanasoski, S., et al. 1995. Genomics. 26: 272-80. PMID: 7601453
5. A CNS-specific POU transcription factor, Brn-2, is required for establishing mammalian neural cell lineages.  |  Fujii, H. and Hamada, H. 1993. Neuron. 11: 1197-206. PMID: 8274283
6. Preferential differentiation of P19 mouse embryonal carcinoma cells into smooth muscle cells. Use of retinoic acid and antisense against the central nervous system-specific POU transcription factor Brn-2.  |  Suzuki, T., et al. 1996. Circ Res. 78: 395-404. PMID: 8593698
7. The different activities of the two activation domains of the Brn-3a transcription factor are dependent on the context of the binding site.  |  Budhram-Mahadeo, V., et al. 1996. J Biol Chem. 271: 9108-13. PMID: 8621561
8. POU domain factor Brn-3b is required for the development of a large set of retinal ganglion cells.  |  Gan, L., et al. 1996. Proc Natl Acad Sci U S A. 93: 3920-5. PMID: 8632990
9. Role of transcription factors Brn-3.1 and Brn-3.2 in auditory and visual system development.  |  Erkman, L., et al. 1996. Nature. 381: 603-6. PMID: 8637595
10. Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursors.  |  Fedtsova, NG. and Turner, EE. 1995. Mech Dev. 53: 291-304. PMID: 8645597
11. A single amino acid change converts an inhibitory transcription factor into an activator.  |  Dawson, SJ., et al. 1996. J Biol Chem. 271: 11631-3. PMID: 8662774
12. Human class III POU genes, POU3F1 and POU3F3, map to chromosomes 1p34.1 and 3p14.2.  |  Sumiyama, K., et al. 1998. Mamm Genome. 9: 180-1. PMID: 9457692

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Brn-2/BRN2/POU3F2 Antibody (B-2) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Brn-2/BRN2/POU3F2 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Brn-2/BRN2/POU3F2 Antibody (B-2) Specifications

Alternate Product Name: POU3F2

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 414-443

Epitope Target: Brn-2

Epitope Species: human

Product Reactivity/Detection: Brn-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Brn-2/BRN2/POU3F2 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent