Brm Antibody (E-1): sc-17828

Brm Antibody (E-1) is a mouse monoclonal IgM antibody that detects Brm protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Brm antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, to suit diverse experimental needs. The Brahma protein (Brm) serves as an ATPase subunit of the Drosophila melanogaster Brm complex, which is closely related to the mammalian SWI/SNF chromatin-remodeling complex, playing a crucial role in gene regulation. Brm functions as a transcriptional activator of Hox genes, interacting with nearly all transcriptionally active chromatin while maintaining a non-overlapping pattern with Polycomb, a known repressor of Hox gene transcription. This unique interaction is vital for proper expression of homeotic genes, as the Brm complex acts as an essential coactivator for the trithorax group protein Zeste, which binds to DNA and activates these genes. Notably, a reduction in Brm function significantly decreases the association of RNA polymerase II with Drosophila salivary gland chromosomes, indicating that chromatin remodeling activity of the Brm complex is fundamental for facilitating transcription by RNA polymerase II. Furthermore, Brm acts as a dominant suppressor of the rough eye phenotype caused by a hypomorphic mutation of Drosophila cyclin E, inhibiting S phase entry by acting downstream of cyclin E protein accumulation. The interaction of the Brm complex with chromatin may also be influenced by BAP111, which is highly associated with the Brm complex in Drosophila embryos through an HMG domain. Brm is predominantly expressed in unfertilized eggs and early embryos, underscoring its importance in early developmental processes.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Brm Antibody (E-1) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-Brm Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Brm Antibody (E-1) Specifications

Alternate Product Name: SMARCA2

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 2-20

Epitope Target: Brm

Epitope Species: human

Product Reactivity/Detection: Brm

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for Brm Antibody (E-1): AC: Agarose, P: Blocking Peptide, X: TransCruz Reagent

The preferred Brm antibody is Brm Antibody (E-6): sc-166579.