Date published: 2025-9-5
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
SMARCA4/Brg1 Antibody (G-7) - Western Blotting - Image 393125
Brg-1 抗体 (G-7) Alexa Fluor® 647: sc-17796 AF647. Jurkat (A), Ramos (B) 和 K-562 (C) 核提取物中 Brg-1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.
Brg-1 抗体 (G-7) Alexa Fluor® 647: sc-17796 AF647. Jurkat (A), Ramos (B) 和 K-562 (C) 核提取物中 Brg-1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.

SMARCA4/Brg1 抗体 (G-7): sc-17796

4.5(22)
寫評論提問

说明书
  • SMARCA4/Brg1 抗体 G-7 是小鼠单克隆 IgG1 κ，SMARCA4/Brg1抗体, 在240篇文献中引用，规格为200 µg/ml
  • 免疫human来源Brg-1近N-terminus的209-296氨基酸
  • SMARCA4/Brg1 抗体 (G-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Brg-1
  • 抗SMARCA4/Brg1抗体（G-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-17796 X, 200 µg/0.1 ml)
  • 关于如何获取SMARCA4/Brg1 (G-7): sc-17796的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 SMARCA4/Brg1 抗体 (G-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SMARCA4/Brg1 抗体 (G-7) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Brg-1抗体（G-7）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Brg-1蛋白 ）。抗Brg-1抗体（G-7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Brg-1，也称为SMARCA4，在SWI-SNF染色质重塑复合物中起着至关重要的作用，该复合物通过ATP依赖性的核小体结构重塑来调节基因表达。这种重塑作用驱动着细胞的各种过程，包括分化、增殖和对外部信号的反应。Brg-1与其他关键的SWI-SNF复合物成分相互作用，例如Brm（SNF2α）、Ini1（整合酶相互作用物1或SNF5）、BAF155和BAF170，形成一个功能核心，增强重塑活性。这些相互作用蛋白稳定复合物并调节活性，从而影响细胞的转录图谱。了解SWI-SNF复合物的动态和组成成分，包括Brg-1，有助于解释基因调控机制及其在包括癌症在内的各种疾病中的潜在作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SMARCA4/Brg1 抗体 (G-7) 参考文献:

  1. 由 SWI/SNF 亚基重构核心染色质重塑复合物。  |  Phelan, ML., et al. 1999. Mol Cell. 3: 247-53. PMID: 10078207
  2. RB 介导的细胞周期停滞需要 BRG-1。  |  Strobeck, MW., et al. 2000. Proc Natl Acad Sci U S A. 97: 7748-53. PMID: 10884406
  3. BRG-1被招募到雌激素反应的启动子中，并与参与组蛋白乙酰化的因子协同作用。  |  DiRenzo, J., et al. 2000. Mol Cell Biol. 20: 7541-9. PMID: 11003650
  4. Brg-1是人体基质金属蛋白酶-2基因最大转录所必需的。  |  Ma, Z., et al. 2004. J Biol Chem. 279: 46326-34. PMID: 15317818
  5. Brg-1靶向新型miR550a-5p/RNF43/Wnt信号轴调节结直肠癌转移。  |  Wang, G., et al. 2016. Oncogene. 35: 651-61. PMID: 25961913
  6. BRG-1靶向新型miR550a-5p/RNF43/Wnt信号轴调节结直肠癌转移。  |  Wang, G., et al. 2017. Oncogene. 36: 5915. PMID: 28846106
  7. 通过恢复 Brg-1 激活 Akt 依赖性 Nrf2/ARE 通路，可缓解高糖诱导的荚膜细胞氧化应激和 ECM 积累。  |  Yan, H., et al. 2021. J Biochem Mol Toxicol. 35: e22672. PMID: 33270355
  8. 酿酒酵母 SNF2/SWI2 和果蝇 brm 基因的人类同源物能增强糖皮质激素受体的转录激活作用。  |  Muchardt, C. and Yaniv, M. 1993. EMBO J. 12: 4279-90. PMID: 8223438
  9. BRG1 含有一个 SWI2/SNF2 家族的保守结构域，是有丝分裂正常生长和转录所必需的。  |  Khavari, PA., et al. 1993. Nature. 366: 170-4. PMID: 8232556
  10. ISWI 是 SWI2/SNF2 ATPase 家族的成员，编码核小体重塑因子的 140 kDa 亚基。  |  Tsukiyama, T., et al. 1995. Cell. 83: 1021-6. PMID: 8521502
  11. 在没有持续 ATP 水解的情况下，人类 SWI/SNF 可维持核小体破坏。  |  Imbalzano, AN., et al. 1996. J Biol Chem. 271: 20726-33. PMID: 8702824
  12. 编码 hSNF2H 的 SMARCA5 的克隆和绘图，hSNF2H 是果蝇 ISWI 的新型人类同源物。  |  Aihara, T., et al. 1998. Cytogenet Cell Genet. 81: 191-3. PMID: 9730600

订购信息

产品名称产品编号规格价格数量收藏夹

SMARCA4/Brg1 抗体 (G-7)

sc-17796
200 µg/ml
$316.00

SMARCA4/Brg1 (G-7): m-IgG Fc BP-HRP 套装

sc-528317
200 µg Ab; 10 µg BP
$354.00

SMARCA4/Brg1 (G-7): m-IgGκ BP-HRP 套装

sc-520661
200 µg Ab, 40 µg BP
$354.00

SMARCA4/Brg1 (G-7): m-IgG1 BP-HRP 套装

sc-542864
200 µg Ab; 20 µg BP
$354.00

SMARCA4/Brg1 抗体 (G-7) X

sc-17796 X
200 µg/0.1 ml
$316.00

SMARCA4/Brg1 抗体 (G-7) AC

sc-17796 AC
500 µg/ml, 25% agarose
$416.00

SMARCA4/Brg1 抗体 (G-7) HRP

sc-17796 HRP
200 µg/ml
$316.00

SMARCA4/Brg1 抗体 (G-7) FITC

sc-17796 FITC
200 µg/ml
$330.00

SMARCA4/Brg1 抗体 (G-7) PE

sc-17796 PE
200 µg/ml
$343.00

SMARCA4/Brg1 抗体 (G-7) Alexa Fluor® 488

sc-17796 AF488
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (G-7) Alexa Fluor® 546

sc-17796 AF546
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (G-7) Alexa Fluor® 594

sc-17796 AF594
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (G-7) Alexa Fluor® 647

sc-17796 AF647
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (G-7) Alexa Fluor® 680

sc-17796 AF680
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (G-7) Alexa Fluor® 790

sc-17796 AF790
200 µg/ml
$357.00

Does any of these anti-Brg1 antibodies react with Xenopus laevis variants? Can you please run the simulation and tell me whether you predict they might react with this species? Thanks. 

Asked by: Anonymous
Thank you for your question. Our Brg1-specific antibodies have only been tested in samples from mouse, rat, and human. We do not have any data supporting reactivity in Xenopus laevis or other species.
Answered by: Tech Support Europe
Date published: 2021-02-09

Dose this Ab recognize (cross react) BRM?

Asked by: Yan1022
Thank you for your question. This antibody is specific for Brg-1, and does not pick up BRM.
Answered by: Tech Service
Date published: 2019-02-22

I need a secondary reagent recommendation to be used with Brg-1 (G-7): sc-17796 mouse monoclonal primary by immunofluorescence?

Asked by: jenni
Thank you for your question. We recommend using one of our kappa light-chain specific fluorescent conjugated Mouse IgG Binding Proteins. We offer m-IgGκ BP's conjugated to FITC, PE, CFL 488, CFL 555 or CFL 594. A link to a complete list of products is provided here. Please contact our Technical Service department for help in choosing the best product for your research. https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2016-12-17

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jenni
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Santa Cruz TS
Date published: 2016-12-14
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_17796, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 78ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Greeat for WBGreat antibody for WB, very clear band, no non-specific band was detected.
Date published: 2024-04-16
Rated 5 out of 5 by from Very sharp and nie results for WBI have eben using this antibody for my Western Blots and it is giving nice sharp results with single bands. I am very happy with it.
Date published: 2023-01-02
Rated 3 out of 5 by from buon funzionamentoho provato l'aliquota gratuita e l'anticorpo ha un buon funzionamento
Date published: 2022-06-01
Rated 3 out of 5 by from Good for WB but not IPSpecific signal for Brg1 in ESCs but it doesn't work for IP from ESCs nuclear extract.
Date published: 2022-04-01
Rated 5 out of 5 by from Excellent for WesternsI performed Western blot for Brg-1/Smarca4 on whole cell extracts for two independent mouse trophoblast stem cell (TSC) lines, and also TSCs differentiated for 2, 4 and 6 days. I used Brg-1 antibody (G-7) diluted 1:1000 in 5% milk TBST. The Western was extremely clean with single bands of the predicted ~182 kDa.
Date published: 2018-03-19
Rated 5 out of 5 by from Brg-1 (G-7) used for western blot applicationBrg-1 (G-7) has been detected on blots containing the lysates from Hek293, MCF7, T47D cell lines using 2.4ug of antibody in 3%BSA, 14ul per 7ml for each blot. Specific sharp bands at expected molecular weight. It is recommended.
Date published: 2018-03-07
Rated 5 out of 5 by from Very good for IPIt works very well for western blot and IP experiments.
Date published: 2018-01-08
Rated 5 out of 5 by from Good quality for ChIPWe used this antibody to immunoprecipitate chromatin-bound BGR1 at the promoter of selected genes. Strong enrichment at promoters of transcriptionally repressed genes.
Date published: 2017-12-01
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_22
  • loc_zh_CN, sid_17796, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
SMARCA4/Brg1 抗体 (G-7) is rated 4.5 out of 5 by 22.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_22
  • loc_zh_CN, sid_17796, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 122ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。