BrdU Antibody (Bu20A): sc-20045
- BrdU Antibody (Bu20A) is a mouse monoclonal IgG1 κ, cited in 12 publications, provided at 200 µg/ml
- raised against bromodeoxyuridine (BrdU) conjugated to a carrier protein
- recommended for detection of BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, by FCM
- recognizes BrdU in denatured DNA of cells labeled with BrdU
- suggested companion product: 5-Bromo-2′ deoxyuridine: sc-290815
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See BrdU (IIB5): sc-32323 for BrdU antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for BrdU Antibody (Bu20A). This work is in progress.
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SEE ALSO...
BrdU Antibody (Bu20A) is a mouse monoclonal IgG1 antibody that detects bromodeoxyuridine in non-species reactive (NSR) samples through flow cytometry (FCM). BrdU, a halogenated pyrimidine analog, plays a crucial role in DNA synthesis as BrdU is incorporated into newly synthesized DNA strands during the S-phase of the cell cycle, making BrdU an invaluable tool for estimating the fraction of cells actively engaged in DNA replication. Quantifying DNA synthesis using anti-BrdU antibody (Bu20A) is essential for various applications, including cancer research, where understanding cell proliferation is key to developing effective therapies. BrdU is instrumental in studying sister chromatid exchange, which can provide insights into genomic stability and DNA repair mechanisms. Incorporation of BrdU into cellular DNA can be achieved through methods such as UV-induced excision-repair synthesis, and subsequent binding of anti-BrdU antibody (Bu20A) to exposed BrdU in single-stranded DNA allows precise detection following hydrochloric acid denaturation or nuclease digestion. Protease antigen recovery is often necessary for tissues or cells fixed with crosslinking agents like formalin, although this may impact BrdU immunodetection specificity.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BrdU Antibody (Bu20A) | sc-20045 | 200 µg/ml | $322.00 | |||
BrdU Antibody (Bu20A) FITC | sc-20045 FITC | 200 µg/ml | $336.00 | |||
BrdU Antibody (Bu20A) PE | sc-20045 PE | 200 µg/ml | $349.00 |