BrdU Antibody (3G26): sc-70443
- BrdU Antibody (3G26) is a mouse monoclonal IgG1, cited in 7 publications, provided at 100 µg/ml
- raised against 5-bromodeoxyuridine (BrdU) conjugated to hemocyanine
- recommended for detection of BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, by IF, IHC(P) and FCM
- recognizes BrdU in denatured DNA of cells labeled with BrdU
- suggested companion product: 5-Bromo-2′ deoxyuridine: sc-290815
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for BrdU Antibody (3G26). This work is in progress.
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SEE ALSO...
BrdU Antibody (3G26) is a mouse monoclonal IgG1 antibody that detects bromodeoxyuridine in non-species reactive samples by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). BrdU, a halogenated pyrimidine analog of thymidine, plays a crucial role in DNA synthesis as BrdU is incorporated into newly synthesized DNA strands during the S-phase of the cell cycle, making BrdU an invaluable tool for estimating the fraction of cells actively engaged in DNA replication. The ability to quantify DNA synthesis through BrdU incorporation is essential for various applications, including cancer research, where understanding cell proliferation is key to developing effective therapies. Anti-BrdU antibody (3G26) binds specifically to exposed BrdU in single-stranded DNA following a hydrochloric acid denaturation step or nuclease digestion, allowing for precise detection of DNA synthesis. Protease antigen recovery is often necessary for tissues or cells fixed with crosslinking agents like formalin, although this may impact the specificity of BrdU immunodetection. BrdU (3G26) antibody′s versatility across multiple applications makes BrdU (3G26) antibody an essential reagent for researchers studying cell proliferation and DNA synthesis dynamics.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BrdU Antibody (3G26) | sc-70443 | 100 µg/ml | $316.00 |