BRDT Antibody (B-11): sc-515674
- BRDT Antibody (B-11) is a mouse monoclonal IgG1 κ BRDT antibody provided at 200 µg/ml
- raised against amino acids 820-851 mapping near the C-terminus of BRDT of human origin
- BRDT Antibody (B-11) is recommended for detection of BRDT of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-BRDT Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for BRDT Antibody (B-11) for WB applications. This reagent is now offered in a bundle with BRDT Antibody (B-11) (see ordering information below).
BRDT Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects BRDT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-BRDT antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BRDT protein, a member of the bromodomain-containing protein family, plays a crucial role in spermatogenesis and is specifically expressed in testis, where BRDT is involved in gene expression regulation during male germ cell development. BRDT′s unique structure, which includes two bromodomains, allows interaction with acetylated lysines on histones, facilitating transcriptional activation of genes essential for spermatogenesis. A PEST sequence in BRDT indicates rapid degradation potential, a process vital for maintaining proper protein levels during dynamic phases of germ cell development. BRDT interacts with other bromodomain-containing proteins, such as BRD2, BRD3, and BRD4, which are implicated in various cellular processes, including chromatin remodeling and transcriptional regulation. Anti-BRDT antibody (B-11) effectively detects this important protein, making BRDT (B-11) antibody a valuable tool for researchers studying male fertility and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BRDT Antibody (B-11) References:
- Structure and ligand of a histone acetyltransferase bromodomain. | Dhalluin, C., et al. 1999. Nature. 399: 491-6. PMID: 10365964
- Expression of cancer-testis antigens in lung cancer: definition of bromodomain testis-specific gene (BRDT) as a new CT gene, CT9. | Scanlan, MJ., et al. 2000. Cancer Lett. 150: 155-64. PMID: 10704737
- Acetylation-dependent chromatin reorganization by BRDT, a testis-specific bromodomain-containing protein. | Pivot-Pajot, C., et al. 2003. Mol Cell Biol. 23: 5354-65. PMID: 12861021
- Identification of unique, differentiation stage-specific patterns of expression of the bromodomain-containing genes Brd2, Brd3, Brd4, and Brdt in the mouse testis. | Shang, E., et al. 2004. Gene Expr Patterns. 4: 513-9. PMID: 15261828
- Molecular cloning and expression of a novel alternative splice variant of BRDT gene. | Zheng, Y., et al. 2005. Int J Mol Med. 15: 315-21. PMID: 15647849
- Identification and characterization of BRDT: A testis-specific gene related to the bromodomain genes RING3 and Drosophila fsh. | Jones, MH., et al. 1997. Genomics. 45: 529-34. PMID: 9367677
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRDT Antibody (B-11) | sc-515674 | 200 µg/ml | $322.00 | |||
BRDT Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-531810 | 200 µg Ab; 20 µg BP | $361.00 | |||
BRDT Antibody (B-11) AC | sc-515674 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BRDT Antibody (B-11) HRP | sc-515674 HRP | 200 µg/ml | $322.00 | |||
BRDT Antibody (B-11) FITC | sc-515674 FITC | 200 µg/ml | $336.00 | |||
BRDT Antibody (B-11) PE | sc-515674 PE | 200 µg/ml | $349.00 | |||
BRDT Antibody (B-11) Alexa Fluor® 488 | sc-515674 AF488 | 200 µg/ml | $364.00 | |||
BRDT Antibody (B-11) Alexa Fluor® 546 | sc-515674 AF546 | 200 µg/ml | $364.00 | |||
BRDT Antibody (B-11) Alexa Fluor® 594 | sc-515674 AF594 | 200 µg/ml | $364.00 | |||
BRDT Antibody (B-11) Alexa Fluor® 647 | sc-515674 AF647 | 200 µg/ml | $364.00 | |||
BRDT Antibody (B-11) Alexa Fluor® 680 | sc-515674 AF680 | 200 µg/ml | $364.00 | |||
BRDT Antibody (B-11) Alexa Fluor® 790 | sc-515674 AF790 | 200 µg/ml | $364.00 |