Date published: 2025-9-5

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BRCA2 抗体 (3D12): sc-293185

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说明书
  • BRCA2 抗体 3D12 是小鼠单克隆 IgG3 κ,BRCA2抗体, 在10篇文献中引用,规格为100 µg/ml
  • 针对与human的BRCA2的氨基酸3319-3418对应的重组蛋白的部分区域
  • 推荐用于 human 来源的BRCA2 WB, IP 和 ELISA检测
  • 关于如何获取BRCA2 (3D12): sc-293185免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是BRCA2 Antibody (3D12) 适用于 WB 应用。 的首选辅助检测试剂,该试剂现与BRCA2 Antibody (3D12) 搭配使用(请参阅下面的订购信息)。

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関連項目

BRCA2抗体(3D12)是一种小鼠单克隆IgG3 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和酶联免疫吸附测定(ELISA)检测人类来源的BRCA2蛋白。BRCA2(3D12)抗体以非结合形式提供。BRCA2蛋白(又称乳腺癌2型易感蛋白)在维持基因组稳定性方面发挥着关键作用,特别是通过参与同源重组修复途径来修复双链DNA断裂。这一功能有助于防止可能导致癌症(特别是乳腺癌和卵巢癌)的突变累积。BRCA2基因位于13q12.3染色体上,编码一种含有多个BRC基序的蛋白质,这些基序对于与Rad51重组酶结合至关重要,而后者是DNA修复过程中的关键参与者。BRCA2基因突变可导致乳腺癌风险显著增加,而具有这些突变的肿瘤通常会表现出野生型等位基因的杂合性缺失,这凸显了BRCA2作为肿瘤抑制剂的作用。抗BRCA2抗体(3D12)有助于深入了解DNA修复机制以及BRCA2突变在癌症生物学中的意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BRCA2 抗体 (3D12) 参考文献:

  1. 基因 BRCA2 突变与食管鳞状细胞癌的风险。  |  Akbari, MR., et al. 2008. Oncogene. 27: 1290-6. PMID: 17724471
  2. BRCA2 功能与中枢神经系统  |  Frappart, PO. and McKinnon, PJ. 2007. Cell Cycle. 6: 2453-7. PMID: 17786054
  3. 疾病家族史和常见 BRCA2 变体对乳腺癌风险的影响。  |  Seymour, IJ., et al. 2008. Oncol Rep. 19: 783-6. PMID: 18288416
  4. BRCA2低效的临床后果。  |  Castells-Roca, L., et al. 2021. NPJ Breast Cancer. 7: 117. PMID: 34504103
  5. 哺乳动物减数分裂中的BRCA2。  |  Zhang, J., et al. 2022. Trends Cell Biol. 32: 281-284. PMID: 34625364
  6. 端粒维持中的BRCA2单倍体不足。  |  Gunnarsdottir, SR., et al. 2021. Genes (Basel). 13: PMID: 35052422
  7. 携带BRCA1或BRCA2突变的预测。  |  Lee, KH. 2023. Ann Transl Med. 11: 271. PMID: 37082677
  8. BRCA2种系突变可识别对PARP抑制剂敏感的胃癌。  |  Petrelli, A., et al. 2023. Cancer Res. 83: 1699-1710. PMID: 37129948
  9. 染色体臂 13q 位点的杂合性缺失模式表明,BRCA2 可能与散发性乳腺肿瘤有关。  |  Kerangueven, F., et al. 1995. Genes Chromosomes Cancer. 13: 291-4. PMID: 7547638
  10. 与染色体 13q12-13 上的 BRCA2 基因有关的一个家族的乳腺癌患者中,野生型等位基因持续丢失。  |  Collins, N., et al. 1995. Oncogene. 10: 1673-5. PMID: 7731724
  11. 乳腺癌易感基因 BRCA2 在染色体 13q12-13 上的定位。  |  Wooster, R., et al. 1994. Science. 265: 2088-90. PMID: 8091231
  12. 男性乳腺癌病例和乳腺癌家族中的 BRCA2 基因突变。  |  Couch, FJ., et al. 1996. Nat Genet. 13: 123-5. PMID: 8673091

订购信息

产品名称产品编号规格价格数量收藏夹

BRCA2 抗体 (3D12)

sc-293185
100 µg/ml
$316.00

BRCA2 (3D12): m-IgG3 BP-HRP 套装

sc-550414
100 µg Ab; 40 µg BP
$354.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with BRCA2 (3D12): sc-293185 antibody?

Asked by: cjMara
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with U2OS + etoposide cell lysate in published research -SCBT QC
Date published: 2023-09-14
Rated 1 out of 5 by from Not clear bandI did not see a clear band by western blot (1:1000 dilution) in ONS-76 medulloblastoma cell lines
Date published: 2021-11-26
Rated 4 out of 5 by from Marca bien la bandaSe puede diferenciar la banda de la proteína y cuantificar, teniendo en cuenta que la transferencia ha de realizarse considerando que es una proteína de alto peso.
Date published: 2019-06-25
Rated 5 out of 5 by from strong bandI have used this antibody,which has a strong band.I will try to buy it next time.
Date published: 2017-05-28
Rated 5 out of 5 by from Great productThe BRCA2 Antibody (3D12): sc-293185 works very well for Western blotting, showing clear and strong band with minimal background.
Date published: 2017-02-07
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BRCA2 抗体 (3D12) is rated 4.0 out of 5 by 5.
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