bradykinin B1 R Antibody (3A2) recommended for detection of bradykinin B1 R of human origin by WB and ELISA

bradykinin B1 R Antibody (3A2): sc-293196

bradykinin B1 R Antibody (3A2) is rated 2.0 out of 5 by 1.
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Datasheets

    • Anti-bradykinin B1 R Antibody (3A2) is a mouse monoclonal IgG2a bradykinin B1 R antibody provided at 100 µg/ml
    • raised against a recombinant protein mapping within amino acids 1-353, representing full length bradykinin B1 R of human origin
    • recommended for detection of bradykinin B1 R of human origin by WB and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of bradykinin B1 R (3A2): sc-293196.
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How much sample do you recommend loading on the gel for Western blot detection using bradykinin B1 R (3A2): sc-293196?

Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2018-05-03
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Rated 2 out of 5 by from okay tested in rat brain homogenates. got non-specific bands.
Date published: 2018-03-13
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