BORIS Antibody (F-1): sc-377085

BORIS Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects BORIS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-BORIS antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Brother of the regulator of imprinted sites (BORIS) is a crucial mammalian transcription factor that shares a paralogous relationship with CCCTC-binding factor (CTCF), a well-known 11 zinc finger protein that plays a vital role in organizing chromatin insulators and regulating imprinted genes. BORIS is a 663 amino acid DNA-binding protein predominantly expressed in the testis, with lower expression levels in the prostate, exhibiting a mutually exclusive pattern that is essential for the resetting of methylation marks during male germ cell differentiation. Abnormal expression of BORIS has been implicated in various cancers, including breast, prostate, ovarian, gastric, liver, endometrial, glial, colon, and esophageal cancers, highlighting BORIS significance in tumorigenesis. Understanding BORIS interactions with specific proteins, such as CTCF, is critical, as these interactions can influence gene expression and chromatin architecture, further emphasizing BORIS importance in both normal development and disease states.

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BORIS Antibody (F-1) References:

1. The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer.  |  Klenova, EM., et al. 2002. Semin Cancer Biol. 12: 399-414. PMID: 12191639
2. Conditional expression of the CTCF-paralogous transcriptional factor BORIS in normal cells results in demethylation and derepression of MAGE-A1 and reactivation of other cancer-testis genes.  |  Vatolin, S., et al. 2005. Cancer Res. 65: 7751-62. PMID: 16140943
3. Reciprocal binding of CTCF and BORIS to the NY-ESO-1 promoter coincides with derepression of this cancer-testis gene in lung cancer cells.  |  Hong, JA., et al. 2005. Cancer Res. 65: 7763-74. PMID: 16140944
4. Genomic and expression profiling of human spermatocytic seminomas: primary spermatocyte as tumorigenic precursor and DMRT1 as candidate chromosome 9 gene.  |  Looijenga, LH., et al. 2006. Cancer Res. 66: 290-302. PMID: 16397242
5. Epigenetic control of CTCFL/BORIS and OCT4 expression in urogenital malignancies.  |  Hoffmann, MJ., et al. 2006. Biochem Pharmacol. 72: 1577-88. PMID: 16854382
6. The potential of BORIS detected in the leukocytes of breast cancer patients as an early marker of tumorigenesis.  |  D'Arcy, V., et al. 2006. Clin Cancer Res. 12: 5978-86. PMID: 17062669
7. Expression of the CTCF-paralogous cancer-testis gene, brother of the regulator of imprinted sites (BORIS), is regulated by three alternative promoters modulated by CpG methylation and by CTCF and p53 transcription factors.  |  Renaud, S., et al. 2007. Nucleic Acids Res. 35: 7372-88. PMID: 17962299
8. Expression of the epigenetic factor BORIS (CTCFL) in the human genome.  |  de Necochea-Campion, R., et al. 2011. J Transl Med. 9: 213. PMID: 22168535
9. BORIS, brother of the regulator of imprinted sites, is aberrantly expressed in hepatocellular carcinoma.  |  Chen, K., et al. 2013. Genet Test Mol Biomarkers. 17: 160-5. PMID: 23237599
10. Brother of Regulator of Imprinted Sites (BORIS) suppresses apoptosis in colorectal cancer.  |  Zhang, Y., et al. 2017. Sci Rep. 7: 40786. PMID: 28098226
11. The prognostic role of BORIS and SOCS3 in human hepatocellular carcinoma.  |  Zhao, R., et al. 2017. Medicine (Baltimore). 96: e6420. PMID: 28328845
12. Association of mutation and expression of the brother of the regulator of imprinted sites (BORIS) gene with breast cancer progression.  |  Akhtar, MS., et al. 2023. Oncotarget. 14: 528-541. PMID: 37235839

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BORIS Antibody (F-1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-BORIS Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BORIS Antibody (F-1) Specifications

Alternate Product Name: BORIS

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 91-210

Epitope Target: BORIS

Epitope Species: human

Product Reactivity/Detection: BORIS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for BORIS Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent