Bora Antibody (H-4): sc-393741

Bora Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bora protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Bora (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bora protein, also known as aurora borealis, is a crucial 559 amino acid protein that plays a significant role in activating Aurora related kinase-1 (ARK-1), a centrosome-associated serine/threonine kinase essential for proper mitotic processes. Bora is primarily localized in the nucleus until mitosis onset, when Bora translocates to the cytoplasm through regulation by activated Cdc2. This translocation enables Bora to bind and activate ARK-1, facilitating centrosome separation, bipolar spindle assembly, and chromosome segregation during cell division. Bora also interacts with polo-like kinase (Plk), which modulates activation loop accessibility for phosphorylation by ARK-1, influencing mitotic entry timing. Disruption of Bora expression leads to multipolar spindle formation during mitosis, a defect also observed when ARK-1 activity is inhibited, highlighting the critical interplay between these proteins in maintaining genomic stability during cell division.

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Bora Antibody (H-4) References:

1. Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis.  |  Berdnik, D. and Knoblich, JA. 2002. Curr Biol. 12: 640-7. PMID: 11967150
2. What's so Bor(a)ing about Aurora-A activation?  |  Wiese, C. and O'Brien, LL. 2006. Dev Cell. 11: 133-4. PMID: 16890151
3. Mitotic activation of the kinase Aurora-A requires its binding partner Bora.  |  Hutterer, A., et al. 2006. Dev Cell. 11: 147-57. PMID: 16890155
4. Roles of Aurora kinases in mitosis and tumorigenesis.  |  Fu, J., et al. 2007. Mol Cancer Res. 5: 1-10. PMID: 17259342
5. Bora and the kinase Aurora a cooperatively activate the kinase Plk1 and control mitotic entry.  |  Seki, A., et al. 2008. Science. 320: 1655-8. PMID: 18566290
6. Bora regulates meiotic spindle assembly and cell cycle during mouse oocyte meiosis.  |  Zhai, R., et al. 2013. Mol Reprod Dev. 80: 474-87. PMID: 23610072
7. Bora downregulation results in radioresistance by promoting repair of double strand breaks.  |  Cairns, J., et al. 2015. PLoS One. 10: e0119208. PMID: 25742493
8. Unique Bora[1]ferrocenophanes with Sterically Protected Boron: A Potential Gateway to Helical Polyferrocenes.  |  Bhattacharjee, H., et al. 2019. Angew Chem Int Ed Engl. 58: 16575-16582. PMID: 31518485
9. BORA regulates cell proliferation and migration in bladder cancer.  |  Cheng, S., et al. 2020. Cancer Cell Int. 20: 290. PMID: 32655322
10. Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry.  |  Tavernier, N., et al. 2021. Nat Commun. 12: 1899. PMID: 33771996
11. The Phospha-Bora-Wittig Reaction.  |  Borys, AM., et al. 2021. J Am Chem Soc. 143: 14065-14070. PMID: 34437805
12. N-Heterocyclic Carbene Stabilized 1-Bora-1,3-butadienes.  |  Chen, C., et al. 2021. J Am Chem Soc. 143: 21312-21320. PMID: 34894685

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bora Antibody (H-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Bora Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bora Antibody (H-4) Specifications

Alternate Product Name: Bora

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-187

Epitope Target: Bora

Epitope Species: human

Product Reactivity/Detection: Bora

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Bora Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin