BoNT/B Antibody (GR-3G7): sc-101376
- BoNT/B Antibody (GR-3G7) is a mouse monoclonal IgG1 κ BoNT/B antibody provided at 200 µg/ml
- raised against BoNT/B of Clostridium botulinum origin
- BoNT/B Antibody (GR-3G7) is recommended for detection of BoNT/B of Clostridium botulinum origin by FCM and ELISA
- Anti-BoNT/B Antibody (GR-3G7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BoNT/B (GR-3G7): sc-101376.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for BoNT/B Antibody (GR-3G7). These reagents are now offered in bundles with BoNT/B Antibody (GR-3G7) (see ordering information below).
BoNT/B Antibody (GR-3G7) is a mouse monoclonal IgG1 kappa light chain antibody that detects the BoNT/B protein of Clostridium botulinum origin by flow cytometry (FCM) and enzyme-linked immunosorbent assay (ELISA). Anti-BoNT/B antibody (GR-3G7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BoNT/B protein, also known as Botulinum neurotoxin type B, is a highly toxic 1,291 amino acid secreted protein that plays a critical role in the pathogenesis of botulism, a rare but severe paralytic illness caused by the bacterium Clostridium botulinum. BoNT/B exists as a disulfide-linked heterodimer composed of a heavy chain and a light chain, which is essential for function. The heavy chain facilitates binding to neuronal synapses, while the light chain acts as an endopeptidase that cleaves specific neuronal proteins, such as VAMP-2, thereby inhibiting neurotransmitter release like acetylcholine. This mechanism leads to the characteristic flaccid paralysis associated with botulism, highlighting the importance of understanding BoNT/B′s structure and function in both clinical and research settings. BoNT/B monoclonal antibody (GR-3G7) enables effective study of this potent neurotoxin′s interactions and effects, contributing to the broader understanding of its role in neuromuscular disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BoNT/B Antibody (GR-3G7) References:
- Crystallographic evidence for doxorubicin binding to the receptor-binding site in Clostridium botulinum neurotoxin B. | Eswaramoorthy, S., et al. 2001. Acta Crystallogr D Biol Crystallogr. 57: 1743-6. PMID: 11679763
- Production and immunogenic efficacy of botulinum tetravalent (A, B, E, F) toxoid. | Torii, Y., et al. 2002. Vaccine. 20: 2556-61. PMID: 12057613
- Synaptotagmins I and II mediate entry of botulinum neurotoxin B into cells. | Dong, M., et al. 2003. J Cell Biol. 162: 1293-303. PMID: 14504267
- Small tripeptide surrogates with low nanomolar affinity as potent inhibitors of the botulinum neurotoxin B metallo-proteolytic activity. | Blommaert, A., et al. 2004. Bioorg Med Chem. 12: 3055-62. PMID: 15142564
- Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence. | Whelan, SM., et al. 1992. Appl Environ Microbiol. 58: 2345-54. PMID: 1514783
- Identification of the amino acid residues rendering TI-VAMP insensitive toward botulinum neurotoxin B. | Sikorra, S., et al. 2006. J Mol Biol. 357: 574-82. PMID: 16430921
- Recombinant C fragment of botulinum neurotoxin B serotype (rBoNTB (HC)) immune response and protection in the rhesus monkey. | Boles, J., et al. 2006. Toxicon. 47: 877-84. PMID: 16730042
- Rapid microplate assay for monitoring botulinum neurotoxin B catalytic activity. | Keller, JE., et al. 1999. J Appl Toxicol. 19 Suppl 1: S13-7. PMID: 10594893
- Buforin I, a natural peptide, inhibits botulinum neurotoxin B activity in vitro. | Garcia, GE., et al. 1999. J Appl Toxicol. 19 Suppl 1: S19-22. PMID: 10594894
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BoNT/B Antibody (GR-3G7) | sc-101376 | 200 µg/ml | $316.00 | |||
BoNT/B Antibody (GR-3G7): m-IgG Fc BP-HRP Bundle | sc-527068 | 200 µg Ab; 10 µg BP | $354.00 | |||
BoNT/B Antibody (GR-3G7): m-IgG1 BP-HRP Bundle | sc-532441 | 200 µg Ab; 20 µg BP | $354.00 | |||
BoNT/B Antibody (GR-3G7) AC | sc-101376 AC | 500 µg/ml, 25% agarose | $416.00 | |||
BoNT/B Antibody (GR-3G7) HRP | sc-101376 HRP | 200 µg/ml | $316.00 | |||
BoNT/B Antibody (GR-3G7) FITC | sc-101376 FITC | 200 µg/ml | $330.00 | |||
BoNT/B Antibody (GR-3G7) PE | sc-101376 PE | 200 µg/ml | $343.00 | |||
BoNT/B Antibody (GR-3G7) Alexa Fluor® 488 | sc-101376 AF488 | 200 µg/ml | $357.00 | |||
BoNT/B Antibody (GR-3G7) Alexa Fluor® 546 | sc-101376 AF546 | 200 µg/ml | $357.00 | |||
BoNT/B Antibody (GR-3G7) Alexa Fluor® 594 | sc-101376 AF594 | 200 µg/ml | $357.00 | |||
BoNT/B Antibody (GR-3G7) Alexa Fluor® 647 | sc-101376 AF647 | 200 µg/ml | $357.00 | |||
BoNT/B Antibody (GR-3G7) Alexa Fluor® 680 | sc-101376 AF680 | 200 µg/ml | $357.00 | |||
BoNT/B Antibody (GR-3G7) Alexa Fluor® 790 | sc-101376 AF790 | 200 µg/ml | $357.00 |