Bob 1 Antibody (Wue-AC5): sc-56817

Bob 1 Antibody (Wue-AC5) is a mouse monoclonal IgG2a antibody that detects Bob 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Bob 1, also known as OBF-1, is a crucial transcriptional coactivator that specifically interacts with the POU domain transcription factors Oct-1 and Oct-2, enhancing their transcriptional activity. This interaction is vital for the regulation of tissue-specific gene expression, particularly in immune cells, as Bob 1 is predominantly expressed in the spleen and peripheral blood leukocytes. The ability of Bob 1 to associate with the octamer motif in the presence of Oct-1 and Oct-2, despite lacking intrinsic DNA-binding capacity, underscores its role as a key co-factor in the transcriptional regulation of immunoglobulin genes and other housekeeping genes. The gene encoding Bob 1 is located on human chromosome 11q23.1, and Bob 1′s function is essential for the proper activation of genes critical for immune response and cellular differentiation. With broad species reactivity and versatility in various applications, anti-Bob 1 antibody (Wue-AC5) serves as an invaluable tool for researchers studying transcriptional regulation and immune cell function.

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Bob 1 Antibody (Wue-AC5) References:

1. Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding.  |  Chang, JF., et al. 1999. J Mol Biol. 288: 941-52. PMID: 10329190
2. Bob1, a Gim5/MM-1/Pfd5 homolog, interacts with the MAP kinase kinase Byr1 to regulate sexual differentiation in the fission yeast, Schizosaccharomyces pombe.  |  Henkel, J., et al. 2001. Differentiation. 67: 98-106. PMID: 11683500
3. Alterations of loci encoding PU.1, BOB1, and OCT2 transcription regulators do not correlate with their suppressed expression in Hodgkin lymphoma.  |  Cavazzini, F., et al. 2005. Cancer Genet Cytogenet. 158: 167-71. PMID: 15796964
4. Bob-1 is expressed in classic Hodgkin lymphoma.  |  Abd El All, HS. 2007. Diagn Pathol. 2: 10. PMID: 17346351
5. Evaluation of CARMA1/CARD11 and Bob1 as candidate genes in common variable immunodeficiency.  |  Tampella, G., et al. 2011. J Investig Allergol Clin Immunol. 21: 348-53. PMID: 21905497
6. Bob1 limits cellular frequency of T-follicular helper cells.  |  Yamashita, K., et al. 2016. Eur J Immunol. 46: 1361-70. PMID: 27080143
7. The Transcriptional Coactivator Bob1 Is Associated With Pathologic B Cell Responses in Autoimmune Tissue Inflammation.  |  Levels, MJ., et al. 2017. Arthritis Rheumatol. 69: 750-762. PMID: 27907250
8. Genetic Interactions Between BOB1 and Multiple 26S Proteasome Subunits Suggest a Role for Proteostasis in Regulating Arabidopsis Development.  |  Silverblatt-Buser, EW., et al. 2018. G3 (Bethesda). 8: 1379-1390. PMID: 29487187
9. The B cell coactivator Bob1 shows DNA sequence-dependent complex formation with Oct-1/Oct-2 factors, leading to differential promoter activation.  |  Gstaiger, M., et al. 1996. EMBO J. 15: 2781-90. PMID: 8654375

The preferred Bob 1 antibody is Bob 1 Antibody (6F10): sc-23932.