Bob 1 Antibody (F-9): sc-365595

Bob 1 Antibody (F-9) is a mouse monoclonal IgG2a antibody that detects Bob 1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Bob 1, also known as OBF-1, is a crucial transcriptional coactivator that specifically interacts with the POU domain proteins Oct-1 and Oct-2, enhancing their transcriptional efficacy. This interaction is vital for the regulation of tissue-specific gene expression, particularly in immune cells, as Bob 1 is predominantly expressed in the spleen and peripheral blood leukocytes. The ability of Bob 1 to associate with the octamer motif in the presence of Oct-1 and Oct-2, despite lacking intrinsic DNA-binding capacity, underscores its role as a key co-factor in the transcriptional activation of genes essential for immune function. The gene encoding Bob 1 is located on human chromosome 11q23.1, and its expression is critical for the proper activation of immunoglobulin promoters, thereby influencing the immune response and maintaining cellular homeostasis. Bob 1 (F-9) antibody serves as an invaluable tool for researchers studying transcriptional regulation and immune cell function.

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Bob 1 Antibody (F-9) References:

1. Bob1 (OCA-B/OBF-1) differential transactivation of the B cell-specific B29 (Ig beta) and mb-1 (Ig alpha) promoters.  |  Malone, CS. and Wall, R. 2002. J Immunol. 168: 3369-75. PMID: 11907094
2. Primary gastric Hodgkin's lymphoma expressing a B-Cell profile including Oct-2 and Bob-1 proteins.  |  Saito, M., et al. 2007. Int J Hematol. 85: 421-5. PMID: 17562619
3. Bob1 maintains T follicular helper cells for long-term humoral immunity.  |  Yanagi, M., et al. 2024. Commun Biol. 7: 185. PMID: 38360857
4. Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation.  |  Galiègue-Zouitina, S., et al. 1995. C R Acad Sci III. 318: 1125-31. PMID: 8574789
5. The B cell transcriptional coactivator BOB1/OBF1 gene fuses to the LAZ3/BCL6 gene by t(3;11)(q27;q23.1) chromosomal translocation in a B cell leukemia line (Karpas 231).  |  Galiègue Zouitina, S., et al. 1996. Leukemia. 10: 579-87. PMID: 8618432
6. The B-cell-specific transcription coactivator OCA-B/OBF-1/Bob-1 is essential for normal production of immunoglobulin isotypes.  |  Kim, U., et al. 1996. Nature. 383: 542-7. PMID: 8849728
7. Cloning and characterization of the murine B-cell specific transcriptional coactivator Bob1.  |  Knoepfel, L., et al. 1996. Biol Chem Hoppe Seyler. 377: 139-45. PMID: 8868069
8. mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p.  |  Hardy, CF., et al. 1997. Proc Natl Acad Sci U S A. 94: 3151-5. PMID: 9096361
9. The B cell-specific coactivator OBF-1 (OCA-B, Bob-1) is inducible in T cells and its expression is dispensable for IL-2 gene induction.  |  Sauter, P. and Matthias, P. 1997. Immunobiology. 198: 207-16. PMID: 9442392
10. Downstream activation of a TATA-less promoter by Oct-2, Bob1, and NF-kappaB directs expression of the homing receptor BLR1 to mature B cells.  |  Wolf, I., et al. 1998. J Biol Chem. 273: 28831-6. PMID: 9786883

The preferred Bob 1 antibody is Bob 1 Antibody (6F10): sc-23932.