Bob 1 Antibody (6F10): sc-23932

Bob 1 Antibody (6F10) is a rat monoclonal IgG2a antibody that detects Bob 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Bob 1 (6F10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bob 1 protein, also known as B cell Oct binding protein 1 or OBF-1, plays a crucial role in the regulation of gene expression, particularly in B cells, by enhancing the transcriptional activity of Oct-1 and Oct-2. This interaction is vital for the proper activation of immunoglobulin gene promoters, which are essential for B cell development and function. Bob 1 is predominantly expressed in the spleen and peripheral blood leukocytes, highlighting its importance in the immune response. Bob 1 protein′s ability to associate with the octamer motif in the presence of Oct-1 and Oct-2, despite lacking intrinsic DNA-binding capacity, underscores its role as a co-factor that facilitates cell-specific transcriptional activation. The gene encoding Bob 1 is located on human chromosome 11q23.1, and Bob 1 expression is tightly regulated, making Bob 1 a significant player in the transcriptional networks that govern B cell biology.

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Bob 1 Antibody (6F10) References:

1. Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration.  |  Tomilin, A., et al. 2000. Cell. 103: 853-64. PMID: 11136971
2. Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator.  |  Lee, L., et al. 2001. Biochemistry. 40: 6580-8. PMID: 11380252
3. Bob1 (OCA-B/OBF-1) differential transactivation of the B cell-specific B29 (Ig beta) and mb-1 (Ig alpha) promoters.  |  Malone, CS. and Wall, R. 2002. J Immunol. 168: 3369-75. PMID: 11907094
4. A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1.  |  Lindner, JM., et al. 2013. Mol Cell Biol. 33: 4628-40. PMID: 24061476
5. A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.  |  Scheidereit, C., et al. 1988. Nature. 336: 551-7. PMID: 2904654
6. Bob1 enhances RORγt-mediated IL-17A expression in Th17 cells through interaction with RORγt.  |  Ikegami, I., et al. 2019. Biochem Biophys Res Commun. 514: 1167-1171. PMID: 31103264
7. The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains.  |  Clerc, RG., et al. 1988. Genes Dev. 2: 1570-81. PMID: 3265124
8. Bob1 maintains T follicular helper cells for long-term humoral immunity.  |  Yanagi, M., et al. 2024. Commun Biol. 7: 185. PMID: 38360857
9. A B-cell coactivator of octamer-binding transcription factors.  |  Gstaiger, M., et al. 1995. Nature. 373: 360-2. PMID: 7779176
10. OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins.  |  Strubin, M., et al. 1995. Cell. 80: 497-506. PMID: 7859290
11. Assignment of the human gene for Oct-binding factor-1 (OBF1), a B-cell-specific coactivator of octamer-binding transcription factors 1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization.  |  Junker, S., et al. 1996. Genomics. 33: 143-5. PMID: 8617501
12. B-cell-specific coactivator OBF-1/OCA-B/Bob1 required for immune response and germinal centre formation.  |  Schubart, DB., et al. 1996. Nature. 383: 538-42. PMID: 8849727

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bob 1 Antibody (6F10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Bob 1 Antibody (6F10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bob 1 Antibody (6F10) Specifications

Alternate Product Name: POU2AF1

Product Clone Name: 6F10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 1-45

Epitope Target: Bob 1

Epitope Species: mouse

Product Reactivity/Detection: Bob 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Bob 1 Antibody (6F10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent