Bob 1 Antibody (6E4): sc-130918
- Bob 1 Antibody (6E4) is a rat monoclonal IgG2a provided at 200 µg/ml
- raised against a recombinant protein corresponding to the N-terminal 45 amino acids of Bob 1 of mouse origin
- recommended for detection of Bob 1 of mouse, rat and human origin by WB, IP and IF
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Bob 1 Antibody (6E4). This work is in progress.
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Bob 1 Antibody (6E4) is a rat monoclonal IgG2a antibody that detects Bob 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Bob 1, also known as OBF-1, plays a crucial role in the regulation of gene expression by specifically interacting with the POU domain transcription factors Oct-1 and Oct-2, enhancing their transcriptional activity. Bob 1 (6E4) antibody is expressed at high levels in immune tissues, such as the spleen and peripheral blood leukocytes, where Bob 1 contributes to the cell-specific activation of Oct-1 and Oct-2, thereby influencing the transcription of immunoglobulin genes and other critical regulatory elements. Bob 1′s ability to associate with the octamer motif in the presence of Oct-1 and/or Oct-2, despite lacking intrinsic DNA-binding capacity, underscores Bob 1′s importance as a co-factor in transcriptional regulation. The gene encoding Bob 1 is located on human chromosome 11q23.1, highlighting Bob 1′s evolutionary conservation and relevance across species. With versatile applications and significant role in gene regulation, anti-Bob 1 antibody (6E4) serves as an essential tool for researchers studying transcriptional mechanisms in various biological contexts.
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Bob 1 Antibody (6E4) References:
- Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. | Tomilin, A., et al. 2000. Cell. 103: 853-64. PMID: 11136971
- Identification of ERdj3 and OBF-1/BOB-1/OCA-B as direct targets of XBP-1 during plasma cell differentiation. | Shen, Y. and Hendershot, LM. 2007. J Immunol. 179: 2969-78. PMID: 17709512
- Expression and prognostic significance of Oct2 and Bob1 in multiple myeloma: implications for targeted therapeutics. | Toman, I., et al. 2011. Leuk Lymphoma. 52: 659-67. PMID: 21438833
- The transcriptional coactivator Bob1 promotes the development of follicular T helper cells via Bcl6. | Stauss, D., et al. 2016. EMBO J. 35: 881-98. PMID: 26957522
- Oct2 and Bob1 are sensitive and specific markers in lineage determination of B cell lymphomas with no expression of conventional B cell markers. | Yin, L., et al. 2016. Histopathology. 69: 775-783. PMID: 27319306
- High frequency of Bob1lo T follicular helper cells in florid reactive follicular hyperplasia. | Matsumiya, H., et al. 2017. Immunol Lett. 191: 23-30. PMID: 28756244
- Agammaglobulinemia with normal B-cell numbers in a patient lacking Bob1. | Kury, P., et al. 2021. J Allergy Clin Immunol. 147: 1977-1980. PMID: 33571536
- Transcriptional Coactivator BOB1 (OBF1, OCA-B) Modulates the Specificity of DNA Recognition by the POU-Domain Factors OCT1 and OCT2 in a Monomeric Configuration. | Nazarov, IB., et al. 2024. Biomolecules. 14: PMID: 38254723
- Heterogeneity of breakpoints at the transcriptional co-activator gene, BOB-1, in lymphoproliferative disease. | Yuille, MA., et al. 1996. Leukemia. 10: 1492-6. PMID: 8751468
- B lymphocytes are impaired in mice lacking the transcriptional co-activator Bob1/OCA-B/OBF1. | Nielsen, PJ., et al. 1996. Eur J Immunol. 26: 3214-8. PMID: 8977324
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bob 1 Antibody (6E4) | sc-130918 | 200 µg/ml | $322.00 |