BNPI Antibody (A-8): sc-377425

BNPI Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects BNPI protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BNPI Antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BNPI protein, also known as brain-specific Na(+)-dependent inorganic phosphate cotransporter, plays a crucial role in glutamatergic neurotransmission by importing phosphate ions into neurons, which are essential for ATP synthesis and other cellular processes. BNPI protein is predominantly expressed in neurons of the cerebral cortex, hippocampus, and cerebellum, with higher expression levels observed in adult brain compared to fetal brain. BNPI localizes almost exclusively to nerve terminals, forming asymmetric excitatory-type synapses and associating preferentially with membranes of small synaptic vesicles. Proper functioning of BNPI is vital for maintaining synaptic transmission and plasticity, as BNPI facilitates glutamate uptake into intracellular vesicles, thereby defining a glutamatergic phenotype in neurons. BNPI regulation of phosphate ion levels is essential for neuronal health and function, making BNPI a significant target for research in neurobiology and related fields.

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BNPI Antibody (A-8) References:

1. Molecular cloning of a novel brain-type Na(+)-dependent inorganic phosphate cotransporter.  |  Aihara, Y., et al. 2000. J Neurochem. 74: 2622-5. PMID: 10820226
2. Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter.  |  Bellocchio, EE., et al. 2000. Science. 289: 957-60. PMID: 10938000
3. Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons.  |  Takamori, S., et al. 2000. Nature. 407: 189-94. PMID: 11001057
4. Transporters in the neurohypophysial neuroendocrine system, with special reference to vesicular glutamate transporters (BNPI and DNPI): a review.  |  Hisano, S. and Nogami, H. 2002. Microsc Res Tech. 56: 122-31. PMID: 11810715
5. Expression of inorganic phosphate/vesicular glutamate transporters (BNPI/VGLUT1 and DNPI/VGLUT2) in the cerebellum and precerebellar nuclei of the rat.  |  Hisano, S., et al. 2002. Brain Res Mol Brain Res. 107: 23-31. PMID: 12414120
6. Optimization of the culture conditions for production of Polyhydroxyalkanoate and its characterization from a new Bacillus cereus sp. BNPI-92 strain, isolated from plastic waste dumping yard.  |  Mohammed, S., et al. 2020. Int J Biol Macromol. 156: 1064-1080. PMID: 31751740
7. De novo assembly and comparative genome analysis for polyhydroxyalkanoates-producing Bacillus sp. BNPI-92 strain.  |  Ebu, SM., et al. 2023. J Genet Eng Biotechnol. 21: 132. PMID: 37991636
8. Regional expression and cellular localization of the Na(+)-dependent inorganic phosphate cotransporter of rat brain.  |  Ni, B., et al. 1995. J Neurosci. 15: 5789-99. PMID: 7643219
9. Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter.  |  Ni, B., et al. 1994. Proc Natl Acad Sci U S A. 91: 5607-11. PMID: 8202535
10. Molecular cloning, expression, and chromosomal localization of a human brain-specific Na(+)-dependent inorganic phosphate cotransporter.  |  Ni, B., et al. 1996. J Neurochem. 66: 2227-38. PMID: 8632143
11. The localization of the brain-specific inorganic phosphate transporter suggests a specific presynaptic role in glutamatergic transmission.  |  Bellocchio, EE., et al. 1998. J Neurosci. 18: 8648-59. PMID: 9786972
12. EAT-4, a homolog of a mammalian sodium-dependent inorganic phosphate cotransporter, is necessary for glutamatergic neurotransmission in caenorhabditis elegans.  |  Lee, RY., et al. 1999. J Neurosci. 19: 159-67. PMID: 9870947

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BNPI Antibody (A-8) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-BNPI Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BNPI Antibody (A-8) Specifications

Alternate Product Name: BNPI

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-55

Epitope Target: BNPI

Epitope Species: human

Product Reactivity/Detection: BNPI

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for BNPI Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin