BNIP-2 Antibody (A-6): sc-377336
- BNIP-2 Antibody (A-6) is a mouse monoclonal IgG1 κ BNIP-2 antibody provided at 200 µg/ml
- raised against amino acids 132-257 mapping within an internal region of BNIP-2 of human origin
- BNIP-2 Antibody (A-6) is recommended for detection of BNIP-2 of human origin by WB, IP, IF and ELISA
- Anti-BNIP-2 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BNIP-2 Antibody (A-6) for WB applications. These reagents are now offered in bundles with BNIP-2 Antibody (A-6) (see ordering information below).
BNIP-2 Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects BNIP-2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-BNIP-2 antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BNIP-2 plays a crucial role in the regulation of apoptosis, functioning as a pro-apoptotic member of the Bcl-2 family. BNIP-2 interacts with other proteins in the family, such as BNIP-1 and BNIP-3, to mediate apoptotic signaling pathways. BNIP-2 enhances the GTPase activity of Cdc42 GTPase-activating protein (Cdc42GAP) through direct binding, which is essential for the hydrolysis of GTP bound to Cdc42. This interaction influences various signaling pathways involving receptor kinases and small GTPases, ultimately impacting cell survival and death. BNIP-2 localizes to the mitochondria, where BNIP-2 associates with Bcl-2 proteins, highlighting a key role in apoptosis regulation, particularly during cellular stress and damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BNIP-2 Antibody (A-6) References:
- Novel BNIP1 variants and their interaction with BCL2 family members. | Zhang, H., et al. 1999. FEBS Lett. 448: 23-7. PMID: 10217402
- Tyrosine phosphorylation of the Bcl-2-associated protein BNIP-2 by fibroblast growth factor receptor-1 prevents its binding to Cdc42GAP and Cdc42. | Low, BC., et al. 1999. J Biol Chem. 274: 33123-30. PMID: 10551883
- Identification of estrogen target genes in human neural cells. | Maggi, A., et al. 2000. J Steroid Biochem Mol Biol. 74: 319-25. PMID: 11162940
- Estrogens, apoptosis and cells of neural origin. | Belcredito, S., et al. 2000. J Neurocytol. 29: 359-65. PMID: 11424952
- Adenovirus E1B 19 kDa and Bcl-2 proteins interact with a common set of cellular proteins. | Boyd, JM., et al. 1994. Cell. 79: 341-51. PMID: 7954800
- Functional complementation of the adenovirus E1B 19-kilodalton protein with Bcl-2 in the inhibition of apoptosis in infected cells. | Chiou, SK., et al. 1994. J Virol. 68: 6553-66. PMID: 8083992
- Functional substitution identifies a cell survival promoting domain common to adenovirus E1B 19 kDa and Bcl-2 proteins. | Subramanian, T., et al. 1995. Oncogene. 11: 2403-9. PMID: 8570192
- The E1B 19K/Bcl-2-binding protein Nip3 is a dimeric mitochondrial protein that activates apoptosis. | Chen, G., et al. 1997. J Exp Med. 186: 1975-83. PMID: 9396766
- Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. | Chen, G., et al. 1999. J Biol Chem. 274: 7-10. PMID: 9867803
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BNIP-2 Antibody (A-6) | sc-377336 | 200 µg/ml | $322.00 | |||
BNIP-2 Antibody (A-6): m-IgGκ BP-HRP Bundle | sc-523288 | 200 µg Ab, 40 µg BP | $361.00 | |||
BNIP-2 Antibody (A-6): m-IgG1 BP-HRP Bundle | sc-533569 | 200 µg Ab; 20 µg BP | $361.00 | |||
BNIP-2 Antibody (A-6) AC | sc-377336 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BNIP-2 Antibody (A-6) HRP | sc-377336 HRP | 200 µg/ml | $322.00 | |||
BNIP-2 Antibody (A-6) FITC | sc-377336 FITC | 200 µg/ml | $336.00 | |||
BNIP-2 Antibody (A-6) PE | sc-377336 PE | 200 µg/ml | $349.00 | |||
BNIP-2 Antibody (A-6) Alexa Fluor® 488 | sc-377336 AF488 | 200 µg/ml | $364.00 | |||
BNIP-2 Antibody (A-6) Alexa Fluor® 546 | sc-377336 AF546 | 200 µg/ml | $364.00 | |||
BNIP-2 Antibody (A-6) Alexa Fluor® 594 | sc-377336 AF594 | 200 µg/ml | $364.00 | |||
BNIP-2 Antibody (A-6) Alexa Fluor® 647 | sc-377336 AF647 | 200 µg/ml | $364.00 | |||
BNIP-2 Antibody (A-6) Alexa Fluor® 680 | sc-377336 AF680 | 200 µg/ml | $364.00 | |||
BNIP-2 Antibody (A-6) Alexa Fluor® 790 | sc-377336 AF790 | 200 µg/ml | $364.00 |