Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BMPR-IA Antibody (7K7): sc-134285. Western blot analysis of BMPR-IA expression in human BMPR-IA transfected (A) and non-transfected (B) 293T whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BMPR-IA CRISPR/Cas9 KO Plasmid (h2): sc-400581-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMPR-IA CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BMPR-IA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMPR-IA Antibody (7K7): sc-134285
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMPR-IA CRISPR/Cas9 KO Plasmid (h2) | sc-400581-KO-2 | 20 µg | $397.00 |