BMP-9 Double Nickase Plasmid (h): sc-402887-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMP-9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BMP-9 Double Nickase Plasmid (h) and BMP-9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GDF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMP-9 Antibody (H-3): sc-514211
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP-9 Double Nickase Plasmid (h) | sc-402887-NIC | 20 µg | $410.00 | |||
BMP-9 Double Nickase Plasmid (h2) | sc-402887-NIC-2 | 20 µg | $410.00 |
Growth differentiation factor 2 (GDF2), which encodes bone morphogenetic protein 9 (BMP-9), is a circulating TGF-β superfamily ligand that signals predominantly through type I receptors ALK1/ACVRL1 and ALK2 with BMPR2, activating SMAD1/5/9 transcriptional programs. In human endothelial and mesenchymal contexts, BMP-9 helps regulate vascular quiescence, angiogenic remodeling, and differentiation-associated gene expression, with pathway cross-talk to MAPK and PI3K signaling influencing cell-state responses. Dysregulation of BMP-9/ALK1 signaling is closely linked to vascular pathobiology, including heritable hemorrhagic telangiectasia and pulmonary arterial hypertension, and altered BMP-9 activity has been studied in fibrosis, inflammation, and tumor microenvironment biology. As a ligand-driven pathway, BMP-9 also provides a tractable axis for interrogating receptor-ligand specificity and downstream SMAD-dependent transcription in relevant primary and engineered cell systems.
BMP-9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GDF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GDF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GDF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GDF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.