BMP-8A CRISPR/Cas9 KO Plasmid (h): sc-406589
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMP-8A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BMP-8A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP-8A CRISPR/Cas9 KO Plasmid (h) | sc-406589 | 20 µg | $397.00 |
Overview
BMP8A encodes bone morphogenetic protein 8A (BMP-8A), a secreted ligand in the TGF-β superfamily that signals through BMP receptors to activate SMAD1/5/9-dependent transcriptional programs. BMP-8A contributes to regulation of developmental patterning, tissue differentiation, and remodeling, and can influence cellular processes such as proliferation, lineage commitment, and extracellular matrix dynamics. As part of the broader BMP signaling network, BMP8A intersects with pathways that govern morphogenesis and organogenesis and can modulate crosstalk with MAPK and other context-specific signaling cascades. Dysregulated BMP pathway activity, including altered ligand availability, is associated with aberrant developmental phenotypes and has been studied in disease-relevant contexts involving fibrosis, skeletal biology, and tumor microenvironment signaling.
BMP-8A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BMP8A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BMP8A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BMP8A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BMP-8A protein expression.
This CRISPR knockout system enables efficient generation of BMP8A-deficient cell models for investigation of BMP-8A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BMP8A exon(s) critical for BMP-8A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BMP8A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BMP-8A CRISPR/Cas9 KO Plasmid (h) and BMP-8A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BMP8A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BMP-8A HDR Plasmid (h) and BMP-8A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BMP8A homology arms to support homology-directed repair at defined BMP8A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.