BMP-4 Double Nickase Plasmid (h): sc-400543-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMP-4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BMP-4 Double Nickase Plasmid (h) and BMP-4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BMP4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMP-4 Antibody (3H2.3): sc-12721
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP-4 Double Nickase Plasmid (h) | sc-400543-NIC | 20 µg | $410.00 | |||
BMP-4 Double Nickase Plasmid (h2) | sc-400543-NIC-2 | 20 µg | $410.00 |
BMP4 encodes bone morphogenetic protein 4 (BMP-4), a secreted TGF-β superfamily ligand that signals through type I/II BMP receptors to activate SMAD1/5/8 and intersect with MAPK pathways. BMP-4 regulates embryonic patterning, mesoderm specification, osteogenic and chondrogenic differentiation, and tissue homeostasis through transcriptional programs controlling proliferation and fate commitment. Dysregulated BMP4/BMP-4 signaling has been implicated in developmental defects, aberrant epithelial–mesenchymal dynamics, fibrosis-related remodeling, and tumor biology, making it a useful entry point for studying context-specific morphogen signaling. In cultured human cells, BMP-4 activity is commonly examined via SMAD phosphorylation, BMP target gene induction (e.g., ID family), and crosstalk with WNT/FGF/NOTCH networks.
BMP-4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BMP4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BMP4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BMP4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BMP4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.