BMP-1 CRISPR/Cas9 KO Plasmid (h): sc-402432
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMP-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BMP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP-1 CRISPR/Cas9 KO Plasmid (h) | sc-402432 | 20 µg | $397.00 |
Overview
BMP1 encodes bone morphogenetic protein 1 (BMP-1), a secreted metalloprotease that functions as a procollagen C-proteinase and a key extracellular matrix (ECM) maturation enzyme. BMP-1 cleaves structural precursors including procollagens and other ECM-associated proteins, coordinating collagen fibrillogenesis, tissue remodeling, and matrix-dependent cell signaling. Through its roles in ECM assembly and turnover, BMP-1 activity intersects with pathways governing cell adhesion, migration, and mechanotransduction in connective tissues. Dysregulated BMP1 function has been associated with heritable and acquired disorders involving abnormal collagen processing and bone/connective tissue integrity, making it relevant to studies of matrix biology and skeletal development.
BMP-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BMP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BMP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BMP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BMP-1 protein expression.
This CRISPR knockout system enables efficient generation of BMP1-deficient cell models for investigation of BMP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BMP1 exon(s) critical for BMP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BMP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BMP-1 CRISPR/Cas9 KO Plasmid (h) and BMP-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BMP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BMP-1 HDR Plasmid (h) and BMP-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BMP1 homology arms to support homology-directed repair at defined BMP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.