BMCP1/KMCP1 Antibody (F-3): sc-376172

BMCP1/KMCP1 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects BMCP1/KMCP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). BMCP1/KMCP1 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BMCP1 protein, also known as uncoupling protein 5 (UCP5) or solute carrier family 25 member 14 (SLC25A14), plays a crucial role in mitochondrial function by facilitating proton transport across the inner mitochondrial membrane, essential for thermogenesis and energy regulation. This 325 amino acid proton channel carrier protein is not coupled to oxidative phosphorylation, allowing proton gradient dissipation and heat generation instead of ATP, particularly important in brown adipose tissue and during cold exposure. Additionally, kidney mitochondrial carrier protein-1 (KMCP1), also referred to as solute carrier family 25 member 30 (SLC25A30), is a highly homologous 291 amino acid multi-pass membrane protein predominantly expressed in kidney cortex and vital for maintaining mitochondrial metabolism, especially under oxidative stress conditions. BMCP1 and KMCP1 interaction highlights the intricate network of mitochondrial carriers regulating cellular energy homeostasis and metabolic responses, making anti-BMCP1/KMCP1 antibody (F-3) an invaluable tool for researchers studying mitochondrial dynamics and related pathologies.

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BMCP1/KMCP1 Antibody (F-3) References:

1. Characterization of novel UCP5/BMCP1 isoforms and differential regulation of UCP4 and UCP5 expression through dietary or temperature manipulation.  |  Yu, XX., et al. 2000. FASEB J. 14: 1611-8. PMID: 10928996
2. Homologues of the uncoupling protein from brown adipose tissue (UCP1): UCP2, UCP3, BMCP1 and UCP4.  |  Bouillaud, F., et al. 2001. Biochim Biophys Acta. 1504: 107-19. PMID: 11239488
3. BMCP1: a mitochondrial uncoupling protein in neurons which regulates mitochondrial function and oxidant production.  |  Kim-Han, JS., et al. 2001. J Neurochem. 79: 658-68. PMID: 11701769
4. Uncoupling proteins--a new family of proteins with unknown function.  |  Erlanson-Albertsson, C. 2002. Nutr Neurosci. 5: 1-11. PMID: 11929192
5. UCP5/BMCP1 transcript isoforms in human skeletal muscle: relationship of the short-insert isoform with lipid oxidation and resting metabolic rates.  |  Yang, X., et al. 2002. Mol Genet Metab. 75: 369-73. PMID: 12051969
6. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.  |  Clark, HF., et al. 2003. Genome Res. 13: 2265-70. PMID: 12975309
7. A new renal mitochondrial carrier, KMCP1, is up-regulated during tubular cell regeneration and induction of antioxidant enzymes.  |  Haguenauer, A., et al. 2005. J Biol Chem. 280: 22036-43. PMID: 15809292
8. The human uncoupling proteins 5 and 6 (UCP5/SLC25A14 and UCP6/SLC25A30) transport sulfur oxyanions, phosphate and dicarboxylates.  |  Gorgoglione, R., et al. 2019. Biochim Biophys Acta Bioenerg. 1860: 724-733. PMID: 31356773
9. Mitochondrial UCP1: Potential thermogenic mechanistic switch for the treatment of obesity and neurodegenerative diseases using natural and epigenetic drug candidates.  |  Reyad-Ul-Ferdous, M., et al. 2024. Phytomedicine. 130: 155672. PMID: 38810549
10. The mitochondrial carrier family of transport proteins: structural, functional, and evolutionary relationships.  |  Kuan, J. and Saier, MH. 1993. Crit Rev Biochem Mol Biol. 28: 209-33. PMID: 8325039
11. Highly conserved charge-pair networks in the mitochondrial carrier family.  |  Nelson, DR., et al. 1998. J Mol Biol. 277: 285-308. PMID: 9514746
12. BMCP1, a novel mitochondrial carrier with high expression in the central nervous system of humans and rodents, and respiration uncoupling activity in recombinant yeast.  |  Sanchis, D., et al. 1998. J Biol Chem. 273: 34611-5. PMID: 9852133

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BMCP1/KMCP1 Antibody (F-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-BMCP1/KMCP1 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BMCP1/KMCP1 Antibody (F-3) Specifications

Alternate Product Name: BMCP1/KMCP1

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 211-295

Epitope Target: BMCP1

Epitope Species: human

Product Reactivity/Detection: all isoforms of BMCP1 (UCP5) and KMCP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for BMCP1/KMCP1 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin