BLOS2 CRISPR/Cas9 KO Plasmid (h): sc-406776
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BLOS2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BLOS2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BLOS2 CRISPR/Cas9 KO Plasmid (h) | sc-406776 | 20 µg | $397.00 |
Overview
BLOC1S2 encodes BLOS2, a core subunit of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) that coordinates endosomal trafficking and cargo sorting. Through BLOC-1 interactions with adaptor protein complexes and cytoskeletal transport machinery, BLOS2 contributes to delivery of membrane proteins to endosomes, lysosomes, and lysosome-related organelles, influencing vesicle maturation and cellular homeostasis. Disruption of BLOC-1 components has been linked to altered neurite outgrowth, synaptic vesicle dynamics, and defects in pigmentation pathways that depend on specialized organelles such as melanosomes. Accordingly, BLOC1S2 is studied in the context of intracellular transport disorders and neurobiology where endolysosomal pathway perturbation can affect signaling and proteostasis.
BLOS2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BLOC1S2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BLOC1S2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BLOC1S2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BLOS2 protein expression.
This CRISPR knockout system enables efficient generation of BLOC1S2-deficient cell models for investigation of BLOS2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BLOC1S2 exon(s) critical for BLOS2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BLOC1S2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BLOS2 CRISPR/Cas9 KO Plasmid (h) and BLOS2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BLOC1S2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BLOS2 HDR Plasmid (h) and BLOS2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BLOC1S2 homology arms to support homology-directed repair at defined BLOC1S2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.