BLNK Antibody (2B11): sc-8003

BLNK Antibody (2B11) is a mouse monoclonal IgG2a kappa light chain antibody that detects BLNK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). BLNK (2B11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BLNK protein, also known as B-cell linker protein, plays a crucial role in B cell receptor (BCR) signaling, which is essential for B cell activation, proliferation, and differentiation. Located primarily in the cytoplasm, BLNK acts as a central hub for signaling pathways initiated by BCR engagement, facilitating the recruitment and activation of downstream effectors such as GRB2, Vav, Nck, and phospholipase C gamma (PLC γ). BLNK′s location in the cell enables signal integration from various kinases, including Syk and Lyn, which are vital for proper B cell development and function. BLNK exists in two isoforms, pp68 and pp70, resulting from alternative splicing of the human BLNK gene transcript, and phosphorylation by Syk is a key regulatory step that enables the activation of downstream signaling cascades. BLNK serves as a significant target for studying B cell biology and related immunological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BLNK Antibody (2B11) References:

1. Btk and BLNK in B cell development.  |  Tsukada, S., et al. 2001. Adv Immunol. 77: 123-62. PMID: 11293115
2. Identification of CMTM7 as a transmembrane linker of BLNK and the B-cell receptor.  |  Miyazaki, A., et al. 2012. PLoS One. 7: e31829. PMID: 22363743
3. The BLNK adaptor protein has a nonredundant role in human B-cell differentiation.  |  Lagresle-Peyrou, C., et al. 2014. J Allergy Clin Immunol. 134: 145-54. PMID: 24582315
4. Involvement of Blnk and Foxo1 in tumor suppression in BCR‑ABL1‑transformed pro‑B cells.  |  Zhang, P., et al. 2021. Oncol Rep. 45: 693-705. PMID: 33416167
5. Silencing BLNK protects against interleukin-1β-induced chondrocyte injury through the NF-κB signaling pathway.  |  Cheng, Y., et al. 2021. Cytokine. 148: 155686. PMID: 34521030
6. Curcumin alleviates experimental colitis via a potential mechanism involving memory B cells and Bcl-6-Syk-BLNK signaling.  |  Wei, SY., et al. 2022. World J Gastroenterol. 28: 5865-5880. PMID: 36353208
7. BLNK negatively regulates innate antifungal immunity through inhibiting c-Cbl-mediated macrophage migration.  |  Yang, YH., et al. 2024. Proc Natl Acad Sci U S A. 121: e2400920121. PMID: 39413134
8. Initiation and processing of signals from the B cell antigen receptor.  |  Reth, M. and Wienands, J. 1997. Annu Rev Immunol. 15: 453-79. PMID: 9143696
9. Molecular mechanisms in B cell antigen receptor signaling.  |  Kurosaki, T. 1997. Curr Opin Immunol. 9: 309-18. PMID: 9203415
10. The complexity of signaling pathways activated by the BCR.  |  DeFranco, AL. 1997. Curr Opin Immunol. 9: 296-308. PMID: 9203421
11. Identification of two tyrosine phosphoproteins, pp70 and pp68, which interact with phospholipase Cgamma, Grb2, and Vav after B cell antigen receptor activation.  |  Fu, C. and Chan, AC. 1997. J Biol Chem. 272: 27362-8. PMID: 9341187
12. BLNK: a central linker protein in B cell activation.  |  Fu, C., et al. 1998. Immunity. 9: 93-103. PMID: 9697839

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BLNK Antibody (2B11) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-BLNK Antibody (2B11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BLNK Antibody (2B11) Specifications

Alternate Product Name: B cell development cytoplasmic LiNKer protein

Product Clone Name: 2B11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 4-205

Epitope Target: BLNK

Epitope Species: human

Product Reactivity/Detection: BLNK p70 and BLNK p68

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for BLNK Antibody (2B11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred BLNK antibody is BLNK Antibody (A-11): sc-514780.