Bim Antibody (H-5): sc-374358

Bim Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody, also known as BCL2L11 antibody, engineered to detect the Bim protein in mouse, rat, and human samples. Bim, or BCL2L11, is a crucial pro-apoptotic member of the Bcl-2 family located primarily in the cytosol and mitochondria, where Bim plays a vital role in regulating programmed cell death by antagonizing anti-apoptotic proteins such as Bcl-XL. Anti-Bim antibody (H-5) targets amino acids 4-195 of the human BimEL isoform, ensuring effective recognition of all three Bim isoforms, BimS, BimL, and BimEL, across multiple species. The localization of Bim is regulated, and under pro-apoptotic conditions, Bim translocates to the mitochondria, where Bim interacts with pro-survival Bcl-2 family members, influencing the intrinsic apoptotic pathway. Bim monoclonal antibody (H-5) is optimized for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), making Bim (H-5) antibody an essential tool for researchers studying apoptosis and cell survival mechanisms. Understanding the cellular localization of Bim is important because Bim′s presence in the mitochondria is critical for the intrinsic apoptotic pathway, influencing key biological processes such as development, immune cell regulation, and cancer progression. Additionally, anti-Bim antibody (H-5) facilitates the investigation of Bim′s regulation, including Bim′s phosphorylation by ERK1/2 and subsequent binding to 14-3-3 proteins, leading to Bim′s sequestration and degradation. These processes are essential for maintaining cellular homeostasis and preventing diseases. By utilizing Bim (H-5) antibody, scientists can gain deeper insights into apoptotic signaling pathways and the delicate balance between cell survival and death, thereby advancing therapeutic research and disease modeling.

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Bim Antibody (H-5) References:

1. The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells.  |  O'Reilly, LA., et al. 2000. Am J Pathol. 157: 449-61. PMID: 10934149
2. Phosphorylation of the pro-apoptotic protein Bim in lymphocytes is associated with protection from apoptosis.  |  Seward, RJ., et al. 2003. Mol Immunol. 39: 983-93. PMID: 12749905
3. Phosphorylation of Bim-EL by Erk1/2 on serine 69 promotes its degradation via the proteasome pathway and regulates its proapoptotic function.  |  Luciano, F., et al. 2003. Oncogene. 22: 6785-93. PMID: 14555991
4. Extracellular signal-regulated kinases 1/2 are serum-stimulated 'Bim(EL) kinases' that bind to the BH3-only protein Bim(EL) causing its phosphorylation and turnover.  |  Ley, R., et al. 2004. J Biol Chem. 279: 8837-47. PMID: 14681225
5. Roles of Bim in apoptosis of normal and Bcr-Abl-expressing hematopoietic progenitors.  |  Kuribara, R., et al. 2004. Mol Cell Biol. 24: 6172-83. PMID: 15226421
6. Bim is an apoptosis sensor that responds to loss of survival signals delivered by epidermal growth factor but not those provided by integrins.  |  Wang, P., et al. 2004. J Biol Chem. 279: 41280-5. PMID: 15292207
7. Cleavage of Mcl-1 by caspases impaired its ability to counteract Bim-induced apoptosis.  |  Herrant, M., et al. 2004. Oncogene. 23: 7863-73. PMID: 15378010
8. The imbalance between Bim and Mcl-1 expression controls the survival of human myeloma cells.  |  Gomez-Bougie, P., et al. 2004. Eur J Immunol. 34: 3156-64. PMID: 15459900
9. Survival factor-induced extracellular signal-regulated kinase phosphorylates BIM, inhibiting its association with BAX and proapoptotic activity.  |  Harada, H., et al. 2004. Proc Natl Acad Sci U S A. 101: 15313-7. PMID: 15486085
10. Generative Design in Building Information Modelling (BIM): Approaches and Requirements.  |  Ma, W., et al. 2021. Sensors (Basel). 21: PMID: 34450882
11. Signal Propagation Within the MCL-1/BIM Protein Complex.  |  Heckmeier, PJ., et al. 2022. J Mol Biol. 434: 167499. PMID: 35189130
12. BCL2L11 Induction Mediates Sensitivity to Src and MEK1/2 Inhibition in Thyroid Cancer.  |  Rose, MM., et al. 2023. Cancers (Basel). 15: PMID: 36672327

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bim Antibody (H-5) has 47 citations in a variety of scientific publications.

Primary antibodies like Anti-Bim Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bim Antibody (H-5) Specifications

Alternate Product Name: Bim

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 4-195

Epitope Target: Bim_EL

Epitope Species: human

Product Reactivity/Detection: Bim_EL, Bim_L and Bim_S

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 47 citations in various scientific publications

Available Conjugates/Forms for Bim Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin