Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Bex1 CRISPR/Cas9 KO Plasmid (m): sc-422657
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Bex1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Bex1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bex1 CRISPR/Cas9 KO Plasmid (m) | sc-422657 | 20 µg | $397.00 |
Overview
BEX1 (brain expressed X-linked 1) encodes a small intracellular protein enriched in neural tissues and implicated in neuronal differentiation, axon guidance, and cell-cycle control. BEX1 participates in signaling networks linked to neurotrophin/NGF responses and can modulate transcriptional programs by interacting with regulatory proteins that influence survival and stress pathways. In the developing and adult nervous system, altered BEX1 expression has been associated with changes in neurite outgrowth and neuronal maintenance, supporting its relevance to neurodevelopmental and neurodegenerative research. Dysregulated BEX1 has also been reported in multiple cancer-related expression datasets, where it has been connected to proliferation and apoptosis phenotypes in tumor biology models.
Bex1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Bex1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Bex1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Bex1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Bex1 protein expression.
This CRISPR knockout system enables efficient generation of Bex1-deficient cell models for investigation of Bex1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Bex1 exon(s) critical for Bex1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Bex1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Bex1 CRISPR/Cas9 KO Plasmid (m) and Bex1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Bex1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Bex1 HDR Plasmid (m) and Bex1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Bex1 homology arms to support homology-directed repair at defined Bex1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.