β-glucuronidase Antibody (E-11): sc-374629
- β-glucuronidase Antibody (E-11) is a mouse monoclonal IgG1 κ β-glucuronidase antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 352-651 mapping at the C-terminus of β-glucuronidase of human origin
- beta-glucuronidase Antibody (E-11) is recommended for detection of β-glucuronidase of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-beta-glucuronidase Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for β-glucuronidase Antibody (E-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with β-glucuronidase Antibody (E-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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β-glucuronidase Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects beta-glucuronidase protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). β-glucuronidase (E-11) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Beta-glucuronidase plays a crucial role in glucuronide hydrolysis, supporting detoxification and excretion of endogenous and exogenous compounds. Beta-glucuronidase deficiencies cause mucopolysaccharidosis type VII (MPS VII), a lysosomal storage disorder marked by glycosaminoglycan buildup, resulting in severe physiological effects. Beta-glucuronidase forms a homotetramer, with each subunit containing a jelly roll barrel, an immunoglobulin constant domain, and a TIM barrel essential for catalytic function. Glu 451 and Glu 540 residues are critical for enzymatic activity, and mutations at these sites can disrupt structure and function. Beta-glucuronidase regulation influences tumorigenesis and cancer invasiveness, making beta-glucuronidase a potential therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
β-glucuronidase Antibody (E-11) References:
- Heparanase and tumor invasion patterns in human oral squamous cell carcinoma xenografts. | Kurokawa, H., et al. 2003. Cancer Sci. 94: 277-85. PMID: 12824922
- Verapamil regulates activity and mRNA-expression of human beta-glucuronidase in HepG2 cells. | Grube, M., et al. 2003. Naunyn Schmiedebergs Arch Pharmacol. 368: 463-9. PMID: 14618298
- Properties of rat and mouse beta-glucuronidase mRNA and cDNA, including evidence for sequence polymorphism and genetic regulation of mRNA levels. | Watson, G., et al. 1985. Gene. 36: 15-25. PMID: 3840761
- Isolation and expression in Escherichia coli of a cDNA clone encoding human beta-glucuronidase. | Guise, KS., et al. 1985. Gene. 34: 105-10. PMID: 3924735
- Beta-glucuronidase in sera of patients with epileptic seizure activity, diabetes and some other disease states. | Varma, R., et al. 1983. Neurosci Lett. 39: 105-11. PMID: 6355907
- Isolation and characterization of the major form of beta-glucuronidase from human seminal plasma. | Gupta, GS. and Singh, GP. 1983. Biochim Biophys Acta. 748: 398-404. PMID: 6416296
- Purification and characterization of microsomal and lysosomal beta-glucuronidase from rat liver by use of immunoaffinity chromatography. | Himeno Mnishimura, Y., et al. 1976. Eur J Biochem. 70: 349-59. PMID: 64358
- Structure of human beta-glucuronidase reveals candidate lysosomal targeting and active-site motifs. | Jain, S., et al. 1996. Nat Struct Biol. 3: 375-81. PMID: 8599764
- Low beta-glucuronidase enzyme activity and mutations in the human beta-glucuronidase gene in mild mucopolysaccharidosis type VII, pseudodeficiency and a heterozygote. | Vervoort, R., et al. 1998. Hum Genet. 102: 69-78. PMID: 9490302
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β-glucuronidase Antibody (E-11) | sc-374629 | 200 µg/ml | $316.00 | |||
β-glucuronidase Antibody (E-11): m-IgGκ BP-HRP Bundle | sc-522796 | 200 µg Ab, 40 µg BP | $354.00 | |||
β-glucuronidase Antibody (E-11) AC | sc-374629 AC | 500 µg/ml, 25% agarose | $416.00 | |||
β-glucuronidase Antibody (E-11) HRP | sc-374629 HRP | 200 µg/ml | $316.00 | |||
β-glucuronidase Antibody (E-11) FITC | sc-374629 FITC | 200 µg/ml | $330.00 | |||
β-glucuronidase Antibody (E-11) PE | sc-374629 PE | 200 µg/ml | $343.00 | |||
β-glucuronidase Antibody (E-11) Alexa Fluor® 488 | sc-374629 AF488 | 200 µg/ml | $357.00 | |||
β-glucuronidase Antibody (E-11) Alexa Fluor® 546 | sc-374629 AF546 | 200 µg/ml | $357.00 | |||
β-glucuronidase Antibody (E-11) Alexa Fluor® 594 | sc-374629 AF594 | 200 µg/ml | $357.00 | |||
β-glucuronidase Antibody (E-11) Alexa Fluor® 647 | sc-374629 AF647 | 200 µg/ml | $357.00 | |||
β-glucuronidase Antibody (E-11) Alexa Fluor® 680 | sc-374629 AF680 | 200 µg/ml | $357.00 | |||
β-glucuronidase Antibody (E-11) Alexa Fluor® 790 | sc-374629 AF790 | 200 µg/ml | $357.00 |