β-Galactosidase Antibody (40-1a): sc-65670

β-Galactosidase Antibody (40-1a) is a mouse monoclonal IgG1 kappa light chain antibody that detects β-Galactosidase across various species by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). β-Galactosidase (40-1a) antibody is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. β-Galactosidase, also known as β-Gal, plays a crucial role in hydrolyzing β-galactosides into monosaccharides, which is essential for carbohydrate metabolism in many organisms. β-Galactosidase facilitates lactose breakdown into glucose and galactose in mammals, providing a vital energy source. β-Galactosidase serves as a reporter gene in molecular biology, enabling researchers to visualize and quantify gene expression through colored or fluorescent products from substrates like X-gal. β-Galactosidase′s tetrameric structure, with four identical subunits, ensures each subunit has an active site for independent catalysis, demonstrating remarkable efficiency in various biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

β-Galactosidase Antibody (40-1a) References:

1. Cell-ELISA using beta-galactosidase conjugated antibodies.  |  Liu, Z., et al. 2000. J Immunol Methods. 234: P153-67. PMID: 10669780
2. Rapid BDNF-induced retrograde synaptic modification in a developing retinotectal system.  |  Du, JL. and Poo, MM. 2004. Nature. 429: 878-83. PMID: 15215865
3. Determination of Acid β-Galactosidase Activity: Methodology and Perspectives.  |  Kwapiszewski, R., et al. 2014. Indian J Clin Biochem. 29: 57-62. PMID: 24478550
4. Alternative splicing of beta-galactosidase mRNA generates the classic lysosomal enzyme and a beta-galactosidase-related protein.  |  Morreau, H., et al. 1989. J Biol Chem. 264: 20655-63. PMID: 2511208
5. A constitutive unregulated expression of β-galactosidase in Lactobacillus fermentum M1.  |  Arukha, AP., et al. 2015. Curr Microbiol. 70: 253-9. PMID: 25319027
6. Gene fusions to lacZ reveal new expression patterns of chimeric genes in transgenic plants.  |  Teeri, TH., et al. 1989. EMBO J. 8: 343-50. PMID: 2656253
7. Beta-galactosidase as a marker in the peripheral and neural tissues of the herpes simplex virus-infected mouse.  |  Ho, DY. and Mocarski, ES. 1988. Virology. 167: 279-83. PMID: 2847416
8. A sensitive micro-immunoassay using beta-galactosidase/anti-beta-galactosidase complexes.  |  Durbin, H. and Bodmer, WF. 1987. J Immunol Methods. 97: 19-27. PMID: 3102611
9. Cloning, sequencing, and expression of cDNA for human beta-galactosidase.  |  Oshima, A., et al. 1988. Biochem Biophys Res Commun. 157: 238-44. PMID: 3143362
10. A new β-galactosidase from Paenibacillus wynnii with potential for industrial applications.  |  Lutz-Wahl, S., et al. 2024. J Dairy Sci. 107: 3429-3442. PMID: 38246536
11. Assignment of human beta-galactosidase-A gene to 3p21.33 by fluorescence in situ hybridization.  |  Takano, T. and Yamanouchi, Y. 1993. Hum Genet. 92: 403-4. PMID: 7693577
12. Grafting genetically modified cells into the rat brain: characteristics of E. coli beta-galactosidase as a reporter gene.  |  Shimohama, S., et al. 1989. Brain Res Mol Brain Res. 5: 271-8. PMID: 2501628

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β-Galactosidase Antibody (40-1a) has 20 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β-Galactosidase Antibody (40-1a) Specifications

Alternate Product Name: β-Galactosidase

Product Clone Name: 40-1a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: β-Galactosidase

Epitope Species: E. coli

Product Reactivity/Detection: β-Galactosidase

Species Reactivity/Detection: E. coli

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for β-Galactosidase Antibody (40-1a): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin