β-Gal Antibody (B-12): sc-377257

β-Gal Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects beta-Gal protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). β-Gal (B-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The human β-galactosidase gene, known as the LacZ gene, is located on chromosome 3p21.33 and encodes a 677 amino acid protein that functions optimally within a pH range of 6 to 8. β-galactosidase plays a crucial role in hydrolyzing terminal galactose residues from glycoproteins and glycolipids, which is essential for various biological processes, including cellular signaling and metabolism. β-galactosidase enzymatic activity is particularly important in lysosomal storage disorders, where deficiency can lead to toxic substrate accumulation. β-galactosidase exists as a tetramer, with each subunit containing an active site capable of independently catalyzing galactose cleavage, and monovalent cations can enhance enzymatic activity. The LacZ gene serves as a reporter gene in molecular biology, enabling researchers to visualize gene expression through colored or fluorescent compounds from substrates like X-gal, which produces a blue color. This versatility makes anti-β-Gal antibody (B-12) an invaluable tool for studying gene expression and protein interactions in various experimental settings.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

β-Gal Antibody (B-12) References:

1. Monoclonal antibodies to Escherichia coli beta-galactosidase and their use for detection and purification of recombinant expression products.  |  Dráber, P., et al. 1992. Hybridoma. 11: 385-90. PMID: 1500073
2. Expression of senescence-associated beta-galactosidase (SA-beta-Gal) by human skin fibroblasts, effect of advanced glycation end-products and fucose or rhamnose-rich polysaccharides.  |  Ravelojaona, V., et al. 2009. Arch Gerontol Geriatr. 48: 151-4. PMID: 18207583
3. Applications of β-gal-III isozyme from Bacillus coagulans RCS3, in lactose hydrolysis.  |  Batra, N., et al. 2011. Int J Biol Macromol. 49: 879-84. PMID: 21855568
4. β-Gal gene expression MRI reporter in melanoma tumor cells. Design, synthesis, and in vitro and in vivo testing of a Gd(III) containing probe forming a high relaxivity, melanin-like structure upon β-Gal enzymatic activation.  |  Arena, F., et al. 2011. Bioconjug Chem. 22: 2625-35. PMID: 22035020
5. Expression of a mouse metallothionein-Escherichia coli beta-galactosidase fusion gene (MT-beta gal) in early mouse embryos.  |  Stevens, ME., et al. 1989. Exp Cell Res. 183: 319-25. PMID: 2504613
6. Alternative splicing of beta-galactosidase mRNA generates the classic lysosomal enzyme and a beta-galactosidase-related protein.  |  Morreau, H., et al. 1989. J Biol Chem. 264: 20655-63. PMID: 2511208
7. Development of a high-throughput β-Gal-based neutralization assay for quantitation of herpes simplex virus-neutralizing antibodies in human samples.  |  Baccari, A., et al. 2016. Vaccine. 34: 3901-6. PMID: 27265458
8. Cloning, sequencing, and expression of cDNA for human beta-galactosidase.  |  Oshima, A., et al. 1988. Biochem Biophys Res Commun. 157: 238-44. PMID: 3143362
9. Synthesis of 'All-Cis' Trihydroxypiperidines from a Carbohydrate-Derived Ketone: Hints for the Design of New β-Gal and GCase Inhibitors.  |  Davighi, MG., et al. 2020. Molecules. 25: PMID: 33023214
10. Predicting the role of β-GAL genes in bean under abiotic stress and genome-wide characterization of β-GAL gene family members.  |  Buttanri, A., et al. 2024. Protoplasma.. PMID: 39441340
11. Assignment of human beta-galactosidase-A gene to 3p21.33 by fluorescence in situ hybridization.  |  Takano, T. and Yamanouchi, Y. 1993. Hum Genet. 92: 403-4. PMID: 7693577
12. A novel panel of monoclonal antibodies against beta-galactosidase of Escherichia coli and its versatility for detection of recombinant expression products.  |  Slavícková, A., et al. 1992. Folia Biol (Praha). 38: 350-7. PMID: 1284241

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β-Gal Antibody (B-12) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-β-Gal Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β-Gal Antibody (B-12) Specifications

Alternate Product Name: GLB1

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 496-575

Epitope Target: β-Gal

Epitope Species: human

Product Reactivity/Detection: β-Gal

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for β-Gal Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin