β-Gal Antibody (148-4): sc-136149

β-Gal Antibody (148-4) is a mouse monoclonal IgM antibody that detects β-galactosidase in mouse, rat, human, bovine, and feline samples through applications such as western blotting (WB) and immunofluorescence (IF). Anti-β-galactosidase antibody (148-4) recognizes β-galactosidase, which plays a crucial role in the hydrolysis of β-galactosides into monosaccharides, a process essential for various biological functions, including lactose metabolism in mammals. β-galactosidase is predominantly located in lysosomes and cytoplasm, where β-galactosidase contributes to the degradation of glycoproteins and glycolipids, thus maintaining cellular homeostasis. β-galactosidase is widely utilized as a reporter gene in molecular biology, allowing researchers to visualize and quantify gene expression through the production of colored or fluorescent products from substrates like X-gal. The tetrameric structure of β-galactosidase, consisting of four identical subunits, is vital for enzymatic activity, as each subunit contains an active site capable of catalyzing the cleavage of terminal galactose. Monovalent cations enhance β-galactosidase activity, indicating the importance of ionic conditions in functional performance. With broad species reactivity and versatile applications, β-Gal monoclonal antibody (148-4) serves as an invaluable tool for researchers studying gene expression and metabolic pathways involving β-galactosidase.

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β-Gal Antibody (148-4) References:

1. A novel pentaglycosylceramide in ostrich liver, IV4-beta-Gal-nLc4Cer, with terminal Gal(beta1-4)Gal, a xenoepitope recognized by human natural antibodies.  |  Bouhours, D., et al. 2000. Glycobiology. 10: 857-64. PMID: 10988247
2. Adenovirus-mediated transfer and expression of beta-gal in injured hippocampus after traumatic brain injury in mice.  |  Kochanek, PM., et al. 2001. J Neurotrauma. 18: 73-82. PMID: 11200251
3. Analysis of fiber-type differences in reporter gene expression of β-gal transgenic muscle.  |  Tai, PW., et al. 2012. Methods Mol Biol. 798: 445-59. PMID: 22130853
4. Digital analysis with droplet-based microfluidic for the ultrasensitive detection of β-gal and AFP.  |  Tian, S., et al. 2018. Talanta. 186: 24-28. PMID: 29784356
5. Deletion of SA β-Gal+ cells using senolytics improves muscle regeneration in old mice.  |  Dungan, CM., et al. 2022. Aging Cell. 21: e13528. PMID: 34904366
6. Fiji-Based Tool for Rapid and Unbiased Analysis of SA-β-Gal Activity in Cultured Cells.  |  Krzystyniak, A., et al. 2023. Biomolecules. 13: PMID: 36830731
7. SA-β-Gal in Kidney Tubules as a Predictor of Renal Outcome in Patients with Chronic Kidney Disease.  |  Esposito, P., et al. 2024. J Clin Med. 13: PMID: 38256456
8. A chalcone-based ESIPT and AIE fluorophore for β-gal imaging in living cells.  |  Hu, Y., et al. 2024. Org Biomol Chem. 22: 1850-1858. PMID: 38345427
9. Visualized Enzyme-Activated Fluorescence Probe for Accurately Detecting β-Gal in Living Cells and BALB/c Nude Mice.  |  Huang, X., et al. 2024. J Fluoresc.. PMID: 38607528
10. Inhibition of mTOR prevents glucotoxicity-mediated increase of SA-beta-gal, p16INK4a, and insulin hypersecretion, without restoring electrical features of mouse pancreatic islets.  |  Guzmán, TJ., et al. 2024. Biogerontology. 25: 819-836. PMID: 38748336

The preferred β-Gal antibody is β-Gal Antibody (B-12): sc-377257.