SAM Transcription Activation: SAM system utilizing dCas9-VP64, MS2-P65-HSF1, and sgRNA for precise gene transcription activation.
beta-catenin Lentiviral Activation Particles (m): sc-419477-LAC
- Target species: mouse
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- beta-catenin Lentiviral Activation Particles (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- beta-catenin Lentiviral Activation Particles (m) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by beta-catenin Lentiviral Activation Plasmid (m) and beta-catenin Lentiviral Activation Plasmid (m2) target distinct regulatory regions of the Ctnnb1 promoter. One or both designs may be available
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: beta-catenin Antibody (E-5): sc-7963
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
beta-catenin Lentiviral Activation Particles (m) | sc-419477-LAC | 200 µl | $455.00 |