Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
β-catenin Antibody (E-2): sc-515074. Western blot analysis of β-catenin expression in HeLa (A), NIH/3T3 (B), A-431 (C), SH-SY5Y (D) and MCF7 (E) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
beta-catenin Double Nickase Plasmid (m): sc-419477-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- beta-catenin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- beta-catenin Double Nickase Plasmid (m) and beta-catenin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ctnnb1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: beta-catenin Antibody (E-5): sc-7963
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
beta-catenin Double Nickase Plasmid (m) | sc-419477-NIC | 20 µg | $410.00 | |||
beta-catenin Double Nickase Plasmid (m2) | sc-419477-NIC-2 | 20 µg | $410.00 |