beta-catenin Antibody (H-1): sc-133240

β-catenin Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 680-781 at the C-terminus of β-catenin, showing reactivity with human, mouse, rat, equine, canine, bovine, and porcine β-catenin in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. β-catenin, also known as CTNNB1, is a multifunctional protein integral to cell adhesion and Wnt signaling pathways. β-catenin undergoes several post-translational modifications, including phosphorylation, ubiquitination, and acetylation, which regulate stability, localization, and activity. Phosphorylation of β-catenin by glycogen synthase kinase 3 (GSK-3) targets β-catenin for ubiquitin-mediated proteasomal degradation, a process critical for suppressing Wnt signaling in the absence of pathway activation. Conversely, when Wnt signaling is active, β-catenin accumulates in the cytoplasm, eventually translocating to the nucleus to regulate gene transcription. These modifications are essential for maintaining cellular homeostasis and controlling processes such as cell proliferation, differentiation, and migration. β-catenin also interacts with E-cadherin at adherens junctions, playing a structural role in maintaining tissue integrity. anti-β-catenin antibody (H-1) provides precise targeting of β-catenin′s C-terminal domain, making this reagent a valuable tool for studying post-translational regulation and functional roles in various cellular contexts.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta-catenin Antibody (H-1) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-beta-catenin Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta-catenin Antibody (H-1) Specifications

Alternate Product Name: β-catenin

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 680-781

Epitope Target: β-catenin

Epitope Species: human

Product Reactivity/Detection: β-catenin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for beta-catenin Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred beta-catenin antibody is beta-catenin Antibody (E-5): sc-7963.