beta Arrestin 1 Double Nickase Plasmid (m): sc-430955-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- beta Arrestin 1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- beta Arrestin 1 Double Nickase Plasmid (m) and beta Arrestin 1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Arrb1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: beta Arrestin 1 Antibody (25-G10): sc-53780
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
beta Arrestin 1 Double Nickase Plasmid (m) | sc-430955-NIC | 20 µg | $410.00 |
Mouse Arrb1 encodes beta Arrestin 1, a multifunctional adaptor that regulates G protein–coupled receptor (GPCR) desensitization, internalization, and trafficking while also scaffolding signaling complexes. Beyond classical receptor uncoupling, beta Arrestin 1 coordinates kinase cascades including MAPK/ERK and can influence PI3K–AKT and NF-κB signaling, linking membrane receptor activity to transcriptional and cytoskeletal responses. Through these pathway interactions, Arrb1 contributes to control of cell migration, inflammatory signaling, and neuronal and immune system responsiveness. Altered arrestin-dependent signaling has been associated with dysregulated receptor signaling networks relevant to cancer biology, metabolic regulation, and neuroinflammatory processes, making Arrb1 a useful node for pathway interrogation in mouse models.
beta Arrestin 1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Arrb1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Arrb1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Arrb1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Arrb1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.