beta Arrestin 1/2 Antibody (21-B1): sc-53781

β-Arrestin-1/2 Antibody (21-B1) is a mouse monoclonal IgG1 antibody engineered to detect both β-Arrestin-1 and β-Arrestin-2 across multiple species including mouse, rat, human, and bovine. Anti-β-Arrestin-1/2 antibody (21-B1) is raised against a synthetic peptide corresponding to the region surrounding Serine 86 to Threonine 98 of human β-Arrestin-1, enabling detection in applications such as western blotting (WB), immunoprecipitation (IP), and immunohistochemistry with paraffin-embedded sections (IHCP). β-Arrestin proteins are pivotal regulators of G protein-coupled receptor (GPCR) signaling, mediating receptor desensitization and internalization while also initiating alternative signaling pathways independent of G proteins. Structurally, both β-Arrestin-1 and β-Arrestin-2 comprise two distinct domains: an N-terminal domain and a C-terminal domain, which together form a bilobal structure essential for their interaction with phosphorylated GPCRs and various signaling proteins. Post-translational modifications, including phosphorylation and ubiquitination, play a crucial role in modulating β-Arrestins′ functionality by regulating their interactions, stability, and localization within the cell. These modifications are vital for the precise control of receptor trafficking and signal termination, thereby influencing diverse physiological processes such as hormone signaling, neurotransmission, and immune responses. Predominantly expressed in spleen and neuronal tissues, β-Arrestin-1 and β-Arrestin-2 are integral to maintaining cellular homeostasis and facilitating appropriate responses to extracellular stimuli. Additionally, β-Arrestins interact with various kinases and adaptor proteins, further emphasizing their importance in intracellular signaling networks. β-Arrestin-1/2 (21-B1) monoclonal antibody offers robust and consistent performance, making this reagent an invaluable tool for researchers studying the structural and functional dynamics of β-Arrestin-mediated signaling pathways.

The preferred β-Arrestin-2 antibody is β-Arrestin-2 Antibody (H-9): sc-13140.