β-Amyloid Antibody (B-4): sc-28365

β-Amyloid Antibody (B-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects beta-Amyloid protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). β-Amyloid (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Beta-Amyloid protein, derived from proteolytic cleavage of Amyloid precursor protein (APP), plays a critical role in Alzheimer′s disease pathogenesis, as accumulation leads to neurotoxic plaque formation in the brain. These plaques are associated with neurodegeneration and cognitive decline, making beta-Amyloid research crucial for understanding and potentially treating Alzheimer′s disease and related disorders. Beta-Amyloid protein′s interaction with other proteins, such as APLP1 and APLP2, which are structurally similar to APP, highlights significance in cellular signaling and membrane dynamics. APLP2, a membrane-bound protein sharing homology with transmembrane-cytoplasmic domains of APP, further emphasizes interconnectedness of these proteins in neurodegenerative diseases.

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β-Amyloid Antibody (B-4) References:

1. Alzheimer's disease: a cell biological perspective.  |  Kosik, KS. 1992. Science. 256: 780-3. PMID: 1589757
2. Inhibition of glycosphingolipid biosynthesis reduces secretion of the beta-amyloid precursor protein and amyloid beta-peptide.  |  Tamboli, IY., et al. 2005. J Biol Chem. 280: 28110-7. PMID: 15923191
3. Relationship Between β-Amyloid and Mitochondrial Dynamics.  |  Kim, DI., et al. 2017. Cell Mol Neurobiol. 37: 955-968. PMID: 27766447
4. The Toxicity and Polymorphism of β-Amyloid Oligomers.  |  Huang, YR. and Liu, RT. 2020. Int J Mol Sci. 21: PMID: 32599696
5. Cross β amyloid assemblies as complex catalytic machinery.  |  Pal, S., et al. 2021. Chem Commun (Camb). 57: 7597-7609. PMID: 34278403
6. Oligomerization by co-assembly of β-amyloid and α-synuclein.  |  Kim, JR. 2023. Front Mol Biosci. 10: 1153839. PMID: 37021111
7. Disrupting Dimeric β-Amyloid by Electric Fields.  |  Vargas-Rosales, PA., et al. 2023. ACS Phys Chem Au. 3: 456-466. PMID: 37780539
8. Amyloid beta/A4 peptide associated with Alzheimer's disease and cerebral amyloid angiopathy.  |  Hirai, S. and Okamoto, K. 1993. Intern Med. 32: 923-5. PMID: 8204971
9. Generation of beta A4 from the amyloid protein precursor and fragments thereof.  |  Dyrks, T., et al. 1993. FEBS Lett. 335: 89-93. PMID: 8243673
10. Paired helical filament-like phosphorylation of tau, deposition of beta/A4-amyloid and memory impairment in rat induced by chronic inhibition of phosphatase 1 and 2A.  |  Arendt, T., et al. 1995. Neuroscience. 69: 691-8. PMID: 8596639
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12. Frameshift mutants of beta amyloid precursor protein and ubiquitin-B in Alzheimer's and Down patients.  |  van Leeuwen, FW., et al. 1998. Science. 279: 242-7. PMID: 9422699

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β-Amyloid Antibody (B-4) has 135 citations in a variety of scientific publications.

Primary antibodies like Anti-β-Amyloid Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β-Amyloid Antibody (B-4) Specifications

Alternate Product Name: APP fragment Amyloid beta 672-714

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 672-714

Epitope Target: β-Amyloid

Epitope Species: human

Product Reactivity/Detection: β-Amyloid and Amyloid A4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 135 citations in various scientific publications

Available Conjugates/Forms for β-Amyloid Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin