beta Actin Antibody (2A3): sc-517582
- beta Actin Antibody (2A3) is a mouse monoclonal IgG1 κ, cited in 108 publications, provided at 200 µg/ml
- raised against a KLH-coupled peptide fragment corresponding to the C-terminal region of β-Actin of human origin
- Anti-beta Actin Antibody (2A3) is recommended for detection of β-Actin of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including and hamster and monkey
- Anti-beta Actin Antibody (2A3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for beta Actin Antibody (2A3). This work is in progress.
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SEE ALSO...
β-Actin Antibody (2A3) is a mouse monoclonal IgG1 kappa light chain antibody raised against a KLH-coupled peptide fragment corresponding to the C-terminal region of human β-Actin. β-Actin monoclonal antibody (2A3) detects β-Actin of mouse, rat, and human origin and is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). β-Actin (2A3) antibody is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. β-Actin is a highly conserved protein that often constitutes up to 50% of total cellular protein in eukaryotic cells. As a major component of the cytoskeleton′s microfilaments, β-Actin plays a critical role in maintaining cell structure, integrity, and motility. Located predominantly in the cytoplasm, β-Actin is involved in essential cellular processes such as muscle contraction, cell division, and the formation of microvilli and the contractile apparatus of muscle cells. In higher eukaryotes, multiple Actin isoforms are encoded by a family of genes, with at least six types present in mammalian tissues that fall into three classes: α-Actin is primarily expressed in muscle tissues, while β-Actin and γ-Actin are the principal constituents of filaments in other cell types. The organization of the Actin cytoskeleton is regulated by members of the small GTPase family, Rho controls the assembly of Actin stress fibers and focal adhesions, Rac regulates Actin filament accumulation at the plasma membrane, and Cdc42 stimulates the formation of filopodia. Understanding β-Actin function and localization is crucial, as β-Actin serves as an important internal control in protein expression studies, and disruptions in β-Actin dynamics are associated with various diseases, including cancer and cardiovascular disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
beta Actin Antibody (2A3) | sc-517582 | 200 µg/ml | $322.00 | |||
beta Actin Antibody (2A3) AC | sc-517582 AC | 500 µg/ml, 25% agarose | $424.00 | |||
beta Actin Antibody (2A3) HRP | sc-517582 HRP | 200 µg/ml | $322.00 | |||
beta Actin Antibody (2A3) FITC | sc-517582 FITC | 200 µg/ml | $336.00 | |||
beta Actin Antibody (2A3) PE | sc-517582 PE | 200 µg/ml | $349.00 | |||
beta Actin Antibody (2A3) Alexa Fluor® 488 | sc-517582 AF488 | 200 µg/ml | $364.00 | |||
beta Actin Antibody (2A3) Alexa Fluor® 546 | sc-517582 AF546 | 200 µg/ml | $364.00 | |||
beta Actin Antibody (2A3) Alexa Fluor® 594 | sc-517582 AF594 | 200 µg/ml | $364.00 | |||
beta Actin Antibody (2A3) Alexa Fluor® 647 | sc-517582 AF647 | 200 µg/ml | $364.00 | |||
beta Actin Antibody (2A3) Alexa Fluor® 680 | sc-517582 AF680 | 200 µg/ml | $364.00 | |||
beta Actin Antibody (2A3) Alexa Fluor® 790 | sc-517582 AF790 | 200 µg/ml | $364.00 |