Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
beta 3 Sodium Potassium ATPase/ATP1B3 Double Nickase Plasmid (h): sc-404570-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- beta 3 Sodium Potassium ATPase/ATP1B3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- beta 3 Sodium Potassium ATPase/ATP1B3 Double Nickase Plasmid (h) and beta 3 Sodium Potassium ATPase/ATP1B3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP1B3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: beta 3 Sodium Potassium ATPase/ATP1B3 Antibody (46): sc-135998
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
beta 3 Sodium Potassium ATPase/ATP1B3 Double Nickase Plasmid (h) | sc-404570-NIC | 20 µg | $410.00 | |||
beta 3 Sodium Potassium ATPase/ATP1B3 Double Nickase Plasmid (h2) | sc-404570-NIC-2 | 20 µg | $410.00 |
ATP1B3 encodes the beta 3 subunit of the Na⁺/K⁺-ATPase, a plasma membrane P-type ATPase complex essential for maintaining Na⁺ and K⁺ gradients that support membrane potential, osmotic balance, and secondary active transport. Beyond ion homeostasis, Na⁺/K⁺-ATPase subunits participate in assembly and trafficking of the pump and can influence signaling outputs linked to cellular adhesion, migration, and growth control. ATP1B3 expression and pump function intersect with processes such as epithelial polarity, neuronal excitability, and stress responses through ion-dependent regulation of transporters and kinases. Dysregulation of Na⁺/K⁺-ATPase subunit composition has been associated with altered proliferative signaling and tissue dysfunction, making ATP1B3 a useful target for mechanistic studies of membrane transport and signaling crosstalk.
beta 3 Sodium Potassium ATPase/ATP1B3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP1B3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP1B3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP1B3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP1B3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.