beta 1 Adrenergic Receptor/ADRB1/β₁-AR CRISPR/Cas9 KO Plasmid (m): sc-419026

Overview

Adrb1 encodes the mouse β1-adrenergic receptor (ADRB1/β1-AR), a G protein–coupled receptor that primarily couples to Gs to stimulate adenylyl cyclase, elevate cAMP, and activate PKA-dependent signaling. In cardiac and other excitable tissues, β1-AR regulates chronotropy, inotropy, and metabolic adaptation through phosphorylation of ion channels and Ca2+ handling proteins, with additional pathway crosstalk through GRK/arrestin-mediated desensitization and receptor internalization. Dysregulated β-adrenergic signaling and altered ADRB1 expression or function are implicated in models of cardiovascular stress responses, heart failure–related remodeling, and arrhythmogenesis, and also influence adipocyte lipolysis and neurohumoral control. As a central node in catecholamine signaling, ADRB1 is widely used to interrogate GPCR signaling dynamics, receptor trafficking, and downstream transcriptional programs.

beta 1 Adrenergic Receptor/ADRB1/β1-AR CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adrb1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adrb1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adrb1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish beta 1 Adrenergic Receptor/ADRB1/β1-AR protein expression.

This CRISPR knockout system enables efficient generation of Adrb1-deficient cell models for investigation of beta 1 Adrenergic Receptor/ADRB1/β1-AR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Adrb1 exon(s) critical for beta 1 Adrenergic Receptor/ADRB1/β1-AR function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Adrb1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by beta 1 Adrenergic Receptor/ADRB1/β1-AR CRISPR/Cas9 KO Plasmid (m) and beta 1 Adrenergic Receptor/ADRB1/β1-AR CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adrb1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by beta 1 Adrenergic Receptor/ADRB1/β1-AR HDR Plasmid (m) and beta 1 Adrenergic Receptor/ADRB1/β1-AR HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adrb1 homology arms to support homology-directed repair at defined Adrb1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.