Bet1 Antibody (16G6): sc-58247
- Bet1 Antibody (16G6) is a mouse monoclonal IgG1 κ Bet1 antibody provided at 200 µg/ml
- raised against full length Bet1 of rat origin
- Bet1 Antibody (16G6) is recommended for detection of Bet1 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-Bet1 Antibody (16G6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Bet1 Antibody (16G6) for WB applications. These reagents are now offered in bundles with Bet1 Antibody (16G6) (see ordering information below).
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Bet1 Antibody (16G6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bet1 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Bet1 (16G6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bet1 protein, also known as Bet1p homolog or rbet1, is a Type IV membrane protein essential for vesicle transport from endoplasmic reticulum to Golgi complex, a critical process for maintaining cellular function and protein processing. Bet1 plays a vital role in forming SNARE complexes, which are necessary for ER-derived vesicle fusion with vesicular tubular clusters, facilitating proper protein and lipid trafficking within cells. Bet1 protein is predominantly localized in vesicular structures concentrated in the peri-Golgi region, but is also distributed throughout the cytoplasm, highlighting its importance in cellular organization and transport mechanisms. Bet1′s interaction with other SNARE proteins underscores its significance in membrane fusion events, making Bet1 a key player in the secretory pathway and a valuable target for research in cellular transport dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Bet1 Antibody (16G6) References:
- Subunit structure of a mammalian ER/Golgi SNARE complex. | Xu, D., et al. 2000. J Biol Chem. 275: 39631-9. PMID: 11035026
- Ykt6 forms a SNARE complex with syntaxin 5, GS28, and Bet1 and participates in a late stage in endoplasmic reticulum-Golgi transport. | Zhang, T. and Hong, W. 2001. J Biol Chem. 276: 27480-7. PMID: 11323436
- The SNARE motif contributes to rbet1 intracellular targeting and dynamics independently of SNARE interactions. | Joglekar, AP., et al. 2003. J Biol Chem. 278: 14121-33. PMID: 12566453
- Multiple cargo binding sites on the COPII subunit Sec24p ensure capture of diverse membrane proteins into transport vesicles. | Miller, EA., et al. 2003. Cell. 114: 497-509. PMID: 12941277
- BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex. | Newman, AP., et al. 1990. Mol Cell Biol. 10: 3405-14. PMID: 2192256
- Mammalian vesicle trafficking proteins of the endoplasmic reticulum and Golgi apparatus. | Hay, JC., et al. 1996. J Biol Chem. 271: 5671-9. PMID: 8621431
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bet1 Antibody (16G6) | sc-58247 | 200 µg/ml | $322.00 | |||
Bet1 Antibody (16G6): m-IgG Fc BP-HRP Bundle | sc-526904 | 200 µg Ab; 10 µg BP | $361.00 | |||
Bet1 Antibody (16G6): m-IgG1 BP-HRP Bundle | sc-532277 | 200 µg Ab; 20 µg BP | $361.00 | |||
Bet1 Antibody (16G6) AC | sc-58247 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Bet1 Antibody (16G6) HRP | sc-58247 HRP | 200 µg/ml | $322.00 | |||
Bet1 Antibody (16G6) FITC | sc-58247 FITC | 200 µg/ml | $336.00 | |||
Bet1 Antibody (16G6) PE | sc-58247 PE | 200 µg/ml | $349.00 | |||
Bet1 Antibody (16G6) Alexa Fluor® 488 | sc-58247 AF488 | 200 µg/ml | $364.00 | |||
Bet1 Antibody (16G6) Alexa Fluor® 546 | sc-58247 AF546 | 200 µg/ml | $364.00 | |||
Bet1 Antibody (16G6) Alexa Fluor® 594 | sc-58247 AF594 | 200 µg/ml | $364.00 | |||
Bet1 Antibody (16G6) Alexa Fluor® 647 | sc-58247 AF647 | 200 µg/ml | $364.00 | |||
Bet1 Antibody (16G6) Alexa Fluor® 680 | sc-58247 AF680 | 200 µg/ml | $364.00 | |||
Bet1 Antibody (16G6) Alexa Fluor® 790 | sc-58247 AF790 | 200 µg/ml | $364.00 |