BDH1 Double Nickase Plasmid (h): sc-405825-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BDH1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BDH1 Double Nickase Plasmid (h) and BDH1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BDH1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BDH1 Antibody (G-5): sc-514413
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BDH1 Double Nickase Plasmid (h) | sc-405825-NIC | 20 µg | $410.00 | |||
BDH1 Double Nickase Plasmid (h2) | sc-405825-NIC-2 | 20 µg | $410.00 |
BDH1 encodes mitochondrial 3-hydroxybutyrate dehydrogenase 1, an NAD+/NADH-dependent enzyme that catalyzes the interconversion of acetoacetate and D-β-hydroxybutyrate in ketone body metabolism. By regulating cellular redox balance and ketone utilization, BDH1 links nutrient availability to mitochondrial energy production and acetyl-CoA supply for downstream biosynthetic and signaling processes. BDH1 activity is therefore relevant to metabolic flexibility across tissues, particularly under fasting, high-fat, or hypoxic conditions that shift reliance toward ketone bodies. Altered BDH1 expression or function has been investigated in contexts of metabolic dysregulation and tumor metabolism, where rewired mitochondrial pathways and redox homeostasis can influence cellular phenotype.
BDH1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BDH1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BDH1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BDH1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BDH1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.