Bcl-x_L Antibody (H-5): sc-8392

Bcl-xL Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bcl-xL of mouse, rat, and human origin through various applications including western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay. Bcl-xL monoclonal antibody (H-5) is offered in both non-conjugated and multiple conjugated forms, such as agarose, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. Bcl-xL, a member of the Bcl-2 family, plays a crucial role in regulating apoptosis, which is essential for maintaining cellular homeostasis and preventing excessive cell death. By inhibiting apoptotic pathways, Bcl-xL contributes to cell survival in response to various stressors, making Bcl-xL particularly important in the context of cancer, where overexpression can lead to tumorigenesis and resistance to therapy. Bcl-xL is expressed as a 233 amino acid protein that shares 43% sequence identity with Bcl-2, and functions by forming heterodimers with pro-apoptotic proteins such as Bax and Bad, thereby modulating their activity and influencing the apoptotic process. Understanding the interactions and regulatory mechanisms of Bcl-xL is vital for developing targeted therapies in cancer treatment, as manipulating expression or function could restore the apoptotic response in cancer cells.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bcl-x_L Antibody (H-5) References:

1. A Bcl-xL timer sets platelet life span.  |  Qi, B. and Hardwick, JM. 2007. Cell. 128: 1035-6. PMID: 17382877
2. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death.  |  Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
3. Deregulated Bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines.  |  Nuñez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
4. Bcl-xL acts as an inhibitor of IP3R channels, thereby antagonizing Ca2+-driven apoptosis.  |  Rosa, N., et al. 2022. Cell Death Differ. 29: 788-805. PMID: 34750538
5. BCL-XL Overexpression Protects Pancreatic β-Cells against Cytokine- and Palmitate-Induced Apoptosis.  |  Perez-Serna, AA., et al. 2023. Int J Mol Sci. 24: PMID: 36982731
6. Bcl-xL is translocated to the nucleus via CtBP2 to epigenetically promote metastasis.  |  Zhang, T., et al. 2023. bioRxiv.. PMID: 37163116
7. Identification of immunosuppressant-induced apoptosis in a murine B-cell line and its prevention by bcl-x but not bcl-2.  |  Gottschalk, AR., et al. 1994. Proc Natl Acad Sci U S A. 91: 7350-4. PMID: 7518929
8. Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death.  |  Yang, E., et al. 1995. Cell. 80: 285-91. PMID: 7834748
9. Interactions among members of the Bcl-2 protein family analyzed with a yeast two-hybrid system.  |  Sato, T., et al. 1994. Proc Natl Acad Sci U S A. 91: 9238-42. PMID: 7937747
10. BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax.  |  Yin, XM., et al. 1994. Nature. 369: 321-3. PMID: 8183370
11. bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death.  |  Boise, LH., et al. 1993. Cell. 74: 597-608. PMID: 8358789
12. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bcl-x_L Antibody (H-5) has 784 citations in a variety of scientific publications.

Primary antibodies like Anti-Bcl-x_L Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bcl-x_L Antibody (H-5) Specifications

Alternate Product Name: BCL2L1

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 165-190

Epitope Target: Bcl-x_L

Epitope Species: human

Product Reactivity/Detection: Bcl-x_L

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 784 citations in various scientific publications

Available Conjugates/Forms for Bcl-x_L Antibody (H-5): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, P: Blocking Peptide, PE: Phycoerythrin, TRITC: Rhodamine