Bcl-2 Antibody (5K140): sc-70411

Bcl-2 Antibody (5K140) is a mouse monoclonal IgG1 antibody that detects Bcl-2 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Bcl-2 is a crucial regulator of apoptosis, playing a significant role in cell survival and tissue homeostasis. Anti-Bcl-2 antibody (5K140) targets this anti-apoptotic, membrane-associated oncoprotein that prevents unnecessary cell death, essential for proper development and protection against foreign pathogens. Bcl-2 achieves protective effects by interacting with other members of the Bcl-2 family, including both death suppressors like Bcl-xL and Mcl-1, and death agonists such as Bax and Bak. Bcl-2′s ability to stabilize the mitochondrial membrane is particularly important, as Bcl-2 interferes with the activation of the cytochrome c/Apaf-1 pathway, thereby preventing the release of pro-apoptotic factors. Additionally, Bcl-2 can undergo post-translational modifications, including phosphorylation, which can further modulate function and interactions. Dysregulation of Bcl-2, often due to mutations in the Bcl-2 gene, is implicated in various cancers, highlighting the importance of Bcl-2 in maintaining normal physiological cell death mechanisms. Bcl-2 monoclonal antibody (5K140) is an invaluable tool for researchers studying apoptosis and its implications in cancer biology.

The preferred Bcl-2 antibody is Bcl-2 Antibody (C-2): sc-7382.