Anti-Bcl-2 Antibody (100) recommended for detection of Bcl-2 of human origin by WB, IP, IF, IHC(P) and FCM

Anti-Bcl-2 Antibody (100): sc-509

Anti-Bcl-2 Antibody (100) is rated 4.7 out of 5 by 34.
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Datasheets

    • Bcl-2 Antibody (100) is a mouse monoclonal IgG1 κ, cited in 989 publications, provided at 200 µg/ml
    • raised against a synthetic peptide corresponding to amino acids 41-54 of human Bcl-2
    • Bcl-2 Antibody (100) is recommended for detection of Bcl-2 of human origin by WB, IP, IF, IHC(P) and FCM; non cross-reactive with Bcl-2 of mouse or rat origin
    • Anti-Bcl-2 Antibody (100) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Bcl-2 (100): sc-509.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Bcl-2 Antibody (100) for WB and IHC(P) applications. This reagent is now offered in a bundle with Bcl-2 Antibody (100) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2016-12-23
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Rated 4 out of 5 by from Good antibody for western blotting Bcl‐2 antibody: sc‐509 is good quality for western blotting (dilute factor: 1:1000)
Date published: 2020-05-25
Rated 4 out of 5 by from WB This antibody worked well for WB at 1:200 in lysis from the breast cancer cells.
Date published: 2019-08-24
Rated 5 out of 5 by from Excellent antibody The antibody shows a strong band at the expected molecular weight.
Date published: 2019-04-10
Rated 5 out of 5 by from 功能很出色! 功能很出色!支持!赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞!
Date published: 2018-06-16
Rated 5 out of 5 by from 功能很出色! 研究生课题与Bax/Bcl-2相关,希望Santa cruz biotechnology能给初实验者一个免费赠送小样的机会,谢谢!
Date published: 2018-02-02
Rated 4 out of 5 by from Decent Bands, Consistent Results Sample was ordered for the purpose of detecting BCL-2 and possible cleavage products. Was pleased to see clear bands at intended size with a 1:500 dilution. Used aliquot multiple times with consistent results.
Date published: 2018-01-04
Rated 5 out of 5 by from NICE results Good antibody.....strong fast signal. .... .
Date published: 2017-12-21
Rated 5 out of 5 by from is wonderful For immunoblotting, I used this mouse sample to dilute with tumor cells in 1:1000 (overnight incubation). Showing obvious bands.
Date published: 2017-10-31
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